Construct: ORF TRCN0000465517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008829.1_s317c1
- Derived from:
- ccsbBroadEn_13764
- DNA Barcode:
- GTTGCCGATGGCTGCTATGAGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465517
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 107986856 | LOC107986856 | uncharacterized LOC107986856 | XR_001745398.2 | 14.9% | (many diffs) | |
| 2 | human | 101927274 | LINC02071 | long intergenic non-protein... | NR_110751.1 | 10.8% | (many diffs) | |
| 3 | human | 107984703 | LOC107984703 | uncharacterized LOC107984703 | NR_148060.1 | 9.7% | (many diffs) | |
| 4 | human | 101929165 | LOC101929165 | uncharacterized LOC101929165 | NR_134476.1 | 8.3% | (many diffs) | |
| 5 | human | 664618 | HSP90AB4P | heat shock protein 90 alpha... | NR_073415.2 | 5.9% | (many diffs) | |
| 6 | human | 105372703 | LOC105372703 | uncharacterized LOC105372703 | XR_001754696.1 | 3.1% | (many diffs) | |
| 7 | human | 112268222 | LOC112268222 | uncharacterized LOC112268222 | XR_002958280.1 | 3% | (many diffs) | |
| 8 | human | 783 | CACNB2 | calcium voltage-gated chann... | XR_001747198.1 | 2.2% | (many diffs) | |
| 9 | human | 54892 | NCAPG2 | non-SMC condensin II comple... | NR_104054.2 | 1.8% | (many diffs) | |
| 10 | human | 57404 | CYP20A1 | cytochrome P450 family 20 s... | NR_163983.1 | 1.8% | (many diffs) | |
| 11 | human | 57404 | CYP20A1 | cytochrome P450 family 20 s... | NR_163984.1 | 1.8% | (many diffs) | |
| 12 | human | 57404 | CYP20A1 | cytochrome P450 family 20 s... | NR_163982.1 | 1.8% | (many diffs) | |
| 13 | human | 57404 | CYP20A1 | cytochrome P450 family 20 s... | NR_163981.1 | 1.7% | (many diffs) | |
| 14 | human | 57404 | CYP20A1 | cytochrome P450 family 20 s... | NR_163985.1 | 1.7% | (many diffs) | |
| 15 | human | 6391 | SDHC | succinate dehydrogenase com... | NR_103459.2 | 1.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 285
- ORF length:
- 219
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgtaaTCCTA GCACTTTGGG AGGCCGAGGC AGGCAGATTG CCTGAACTCA 121 GGAGTTCAAG ACCAGCCTGG ACAACACGGC GAAACCCCGT CTCTACTAAA ATTCAAAAAA 181 TTAGCCAGGC ATGGCAACGT GTACCTGTAA TCCCAGCTAC TAGGGAGGCT GAGGCAGGAA 241 AATTGCTTGA ACCGCTCAGA CAGAGCAAGA CTCCGTCTTT AAAATACCCA ACTTTCTTGT 301 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 361 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 421 GAAAGGACGA GTTGCCGATG GCTGCTATGA GATTACGCGT TAAGTCgaca atcaacctct 481 ggattacaaa atttgtgaaa gatt