Construct: ORF TRCN0000465522
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004944.1_s317c1
- Derived from:
- ccsbBroadEn_10512
- DNA Barcode:
- GAATTGCGCGCTTCAACACGCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL23AP32 (56969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465522
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6147 | RPL23A | ribosomal protein L23a | NM_000984.6 | 77.1% | 71.1% | (many diffs) |
| 2 | human | 56969 | RPL23AP32 | ribosomal protein L23a pseu... | NR_002229.2 | 62.7% | 1_124del;382C>T;515_620del | |
| 3 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026973.1 | 49.9% | (many diffs) | |
| 4 | human | 649946 | RPL23AP64 | ribosomal protein L23a pseu... | NR_003040.2 | 47.4% | (many diffs) | |
| 5 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_000029.3 | 28.4% | (many diffs) | |
| 6 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024529.1 | 25.2% | (many diffs) | |
| 7 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026982.1 | 24.2% | (many diffs) | |
| 8 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024528.1 | 23.6% | (many diffs) | |
| 9 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026981.1 | 23% | (many diffs) | |
| 10 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026972.1 | 18.2% | (many diffs) | |
| 11 | human | 388574 | RPL23AP87 | ribosomal protein L23a pseu... | NR_029406.1 | 15.4% | (many diffs) | |
| 12 | human | 644128 | RPL23AP53 | ribosomal protein L23a pseu... | NR_003572.2 | 5.8% | (many diffs) | |
| 13 | mouse | 268449 | Rpl23a | ribosomal protein L23A | NM_207523.2 | 71.7% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 459
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaccgaaa gtgaagaagg aagctcctgg cccgccTAAA GCTGAAGCCA 121 AAGCAAAGGC TTTAAAGGCC AAGAAGGTAG TGTTGAAAGG TGTCCACGGC CACAAAAAAA 181 AGAAGATCCG CATGTCACCC ACCTTCCAGC GGCCCAAGAC ACTGAGACTC TGGAGGCCGC 241 CCAGATATCC TCGGAAGACC ACCCCCAGGA GAAACAAGCT TGACCACTAT GCTATCATCA 301 AGTTTCCTCT GACCACTGAG TTTGCTATGA AGAAGATAAA AGACAACAAC ACCCTTGTGT 361 TCACTGTGGA TGTTAAAGCC AACAAGCACC AGATCAAACA GGCTGTGAAG AAGCTCTGTG 421 ACATTGATGG GGCCAAGGTC AACACCCTGA TGGAGAGATT GCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGAGAATTG CGCGCTTCAA CACGCTATAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt