Construct: ORF TRCN0000465523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011528.1_s317c1
- Derived from:
- ccsbBroadEn_09986
- DNA Barcode:
- GTACGGACATCCGCCAAAATCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNPLA1 (285848)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465523
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285848 | PNPLA1 | patatin like phospholipase ... | NM_173676.2 | 99.9% | 99.7% | 983C>A |
| 2 | human | 285848 | PNPLA1 | patatin like phospholipase ... | NM_001145716.2 | 97.9% | 97.7% | 219_245del;1010C>A |
| 3 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_011514520.2 | 91.5% | 91.4% | 983C>A;1312_1431del |
| 4 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_017010778.1 | 89.8% | 89.7% | 219_245del;1010C>A;1339_1458del |
| 5 | human | 285848 | PNPLA1 | patatin like phospholipase ... | NM_001145717.1 | 82% | 81.9% | 1_285del;1268C>A |
| 6 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_017010777.2 | 80.7% | 80.5% | 1_285del;504_530del;1295C>A |
| 7 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_011514519.2 | 76.3% | 76.2% | 1_285del;1268C>A;1597_1716del |
| 8 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_017010776.1 | 75.1% | 75% | (many diffs) |
| 9 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_017010779.1 | 74.3% | 74.2% | 0_1ins246;737C>A;1066_1185del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1380
- ORF length:
- 1311
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgcagatg atgaggcagt ttctgtaccg ggtcctgccc gaggactcct 121 acaaggtcac cacggggaag ctccatgtga gcctcacccg cttaacggac ggggagaatg 181 tggtggtttc agagttcacg tccaaggagg agctcattga ggccctatac tgcagctgct 241 tcgtcccggt gtactgtggc ctcatccccc cgacttaccg cggtgtgagg tacatcgatg 301 ggggcttcac gggcatgcag ccctgtgcct tctggaccga cgccatcacc atctccacct 361 tcagtgggca gcaggacatc tgtccccggg actgcccggc catcttccac gacttccgca 421 tgttcaactg ctccttccag ttctccctgg agaacatcgc caggatgacc cacgcattgt 481 tccccccgga cctggtgatc ctgcacgatt actactaccg agggtacgag gatgcagttt 541 tgtacttgag gcggctgaat gctgtttatc ttaattcttc ctccaagaga gtgattttcc 601 cccgggtgga agtgtactgc cagatagaac tcgcccttgg caatgagtgc cctgaacgca 661 gtcaaccaag ccttcgagca cggcaggcca gtctggaagg agccacacaa cctcacaagg 721 agtgggttcc caaaggggat ggaaggggca gccatggtcc gcctgtgtcc caacctgtgc 781 agacacttga attcacatgc gagtcacctg tttcagcacc agtctctcca cttgagcagc 841 cacctgcaca gccactggcc tcttcaactc cactttctct aagtggcatg ccacctgtat 901 cattcccagc tgtgcacaag ccacccagct ccacacctgg ttcatcactg cccaccccac 961 cacctggact gtcacctctg tcacctcagc agcaggtaca accgtctgga tcaccagcca 1021 gatccctaca ctctcaggca cccacttcac acaggccatc cctggggcct tcaactgtgg 1081 gggcacctca aacactgccc cgaagttctc tttcagcctt ccctgcTCAG CCACCTGTGG 1141 AGGAACTAGG CCAAGAACAG CCCCAAGCTG TAGCTCTTCT TGTCTCTTCA AAACCAAAAA 1201 GCGCCGTGCC TCTGGTTCAT GTGAAGGAAA CCGTCAGCAA GCCTTATGTA ACGGAGAGCC 1261 CTGCTGAAGA CTCAAACTGG GTGAATAAGG TCTTCAAGAA GAACAAGCAA AAGACAAGTG 1321 GCACCAGAAA AGGCTTCCCA AGACATTCGG GATCCAAAAA ACCAAGCAGC AAAGTGCAGT 1381 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGAGTACG GACATCCGCC AAAATCTACA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt