Construct: ORF TRCN0000465527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001004.1_s317c1
- Derived from:
- ccsbBroadEn_11805
- DNA Barcode:
- ACCATTAGCTTTGGCCTCTAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HACL1 (26061)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NM_001284416.2 | 23.4% | 21.3% | (many diffs) |
| 2 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NM_001284415.2 | 22% | 20.5% | (many diffs) |
| 3 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NM_012260.4 | 20.5% | 18.4% | 308_380del;431_1734del |
| 4 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NR_104315.2 | 19.5% | 1_106del;464_1830del | |
| 5 | human | 26061 | HACL1 | 2-hydroxyacyl-CoA lyase 1 | NM_001284413.2 | 18.4% | 14.8% | (many diffs) |
| 6 | mouse | 56794 | Hacl1 | 2-hydroxyacyl-CoA lyase 1 | NM_019975.3 | 18.4% | 17.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggacagtaac ttcgcagagc gcagcgagga gcaggtgtct ggtgctaaag 121 tcatcgctca ggccctgaaa acgcaagatg tggagtacat atttGGCATC GTAGGCATCC 181 CAGTGACCGA AATCGCCATT GCTGCCCAGC AGCTAGGCAT CAAGTACATC GGGATGAGGA 241 ATGAGCAAGC GGCTTGTTAT GCTGCCTCCG CGATTGGATA TCTGACAAGC AGGCCAGGAG 301 TCTGCCTTGT TGTTTCTGGC CCAGGTCTCA TCCATGCCTT GGGCGGTATG GCAAATGCAA 361 ACATGAACTG CTGGTTGAAG CTTGTAGATT ATATACCAAG TTCTCTGCCC GCCCAAGCAG 421 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 541 GGCTTTATAT ATCTTGTGGA AAGGACGAAC CATTAGCTTT GGCCTCTAAT ATACGCGTTA 601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt