Construct: ORF TRCN0000465528
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016508.1_s317c1
- Derived from:
- ccsbBroadEn_16051
- DNA Barcode:
- TCTGCTTATGCAGCGCAATCAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGS8 (85397)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465528
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85397 | RGS8 | regulator of G protein sign... | NM_001102450.2 | 100% | 100% | |
| 2 | human | 85397 | RGS8 | regulator of G protein sign... | NM_001369564.2 | 100% | 100% | |
| 3 | human | 85397 | RGS8 | regulator of G protein sign... | XM_005245555.4 | 100% | 100% | |
| 4 | human | 85397 | RGS8 | regulator of G protein sign... | XM_011510090.2 | 100% | 100% | |
| 5 | human | 85397 | RGS8 | regulator of G protein sign... | XM_017002635.1 | 100% | 100% | |
| 6 | human | 85397 | RGS8 | regulator of G protein sign... | XM_017002637.2 | 100% | 100% | |
| 7 | human | 85397 | RGS8 | regulator of G protein sign... | XM_011510091.3 | 97.2% | 94.5% | (many diffs) |
| 8 | human | 85397 | RGS8 | regulator of G protein sign... | NM_033345.3 | 89.1% | 87.3% | (many diffs) |
| 9 | human | 85397 | RGS8 | regulator of G protein sign... | XM_011510089.3 | 89.1% | 87.3% | (many diffs) |
| 10 | human | 85397 | RGS8 | regulator of G protein sign... | XM_017002631.2 | 89.1% | 87.3% | (many diffs) |
| 11 | human | 85397 | RGS8 | regulator of G protein sign... | XM_017002632.1 | 89.1% | 87.3% | (many diffs) |
| 12 | human | 85397 | RGS8 | regulator of G protein sign... | XM_017002633.1 | 89.1% | 87.3% | (many diffs) |
| 13 | human | 85397 | RGS8 | regulator of G protein sign... | XM_017002634.1 | 89.1% | 87.3% | (many diffs) |
| 14 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | NM_026380.3 | 90.7% | 98.3% | (many diffs) |
| 15 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529818.3 | 90.7% | 98.3% | (many diffs) |
| 16 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529819.3 | 90.7% | 98.3% | (many diffs) |
| 17 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529820.3 | 90.7% | 98.3% | (many diffs) |
| 18 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529821.3 | 90.7% | 98.3% | (many diffs) |
| 19 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | NM_001347115.1 | 88.3% | 93.8% | (many diffs) |
| 20 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529822.3 | 88.3% | 93.8% | (many diffs) |
| 21 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529824.3 | 88.3% | 93.8% | (many diffs) |
| 22 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529825.3 | 88.3% | 93.8% | (many diffs) |
| 23 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529826.3 | 88.3% | 93.8% | (many diffs) |
| 24 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529827.3 | 88.3% | 93.8% | (many diffs) |
| 25 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529828.3 | 88.3% | 93.8% | (many diffs) |
| 26 | mouse | 67792 | Rgs8 | regulator of G-protein sign... | XM_006529829.3 | 72.9% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 606
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccttactg atgccacgca ggaacaaagg gatgaggact cgactgggat 121 gcctgtctca caagtcagac tcgtgtagtg atttcacagc tattcttcca gacaaaccca 181 accgcgctct caagagatta tcgacagaag aagctacgag gtgggcagat tcctttgatg 241 tgcttctctc tcataagtat ggggtggctg cattccgtgc cTTCTTGAAG ACGGAGTTCA 301 GTGAGGAGAA CCTGGAATTC TGGTTGGCCT GTGAGGAGTT CAAGAAGACC AGGTCAACTG 361 CAAAACTGGT CTCTAAGGCC CATAGGATCT TTGAGGAGTT TGTGGATGTG CAGGCTCCAC 421 GGGAGGTAAA CATTGACTTC CAGACCCGAG AAGCCACGAG GAAGAACCTG CAGGAGCCAT 481 CCCTGACTTG CTTTGACCAA GCCCAAGGAA AAGTACACAG CCTCATGGAG AAAGACTCTT 541 ACCCCAGGTT CCTGAGGTCC AAAATGTACT TAGATCTGCT GTCCCAAAGC CAGAGGAGGC 601 TCAGTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 661 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 721 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTGCTTAT GCAGCGCAAT CAGACACGCG 781 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt