Construct: ORF TRCN0000465538
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003223.1_s317c1
- Derived from:
- ccsbBroadEn_00947
- DNA Barcode:
- AATCGGGACTGATGGGCAGCATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VWA5A (4013)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465538
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4013 | VWA5A | von Willebrand factor A dom... | NM_198315.2 | 100% | 100% | |
| 2 | human | 4013 | VWA5A | von Willebrand factor A dom... | NM_001130142.2 | 52.7% | 52.7% | 1246_2358del |
| 3 | human | 4013 | VWA5A | von Willebrand factor A dom... | NM_014622.4 | 52.7% | 52.7% | 1246_2358del |
| 4 | human | 4013 | VWA5A | von Willebrand factor A dom... | XM_011542828.2 | 51.7% | 51.7% | 1_48del;1294_2406del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1311
- ORF length:
- 1245
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcacttctgt ggcctactca ccctccaccg ggagccagtg ccgctgaaga 121 gtatctctgt gagcgtgaac atttacgagt ttgtggctgg tgtgtctgca actttgaact 181 acgagaatga ggagaaagtt cctttggagg ccttctttgt gttccccatg gatgaagact 241 ctgctgttta cagctttgag gccttggtgg atgggaagaa aattgtagca gaattacaag 301 acaagatgaa ggcccgcacc aactatgaga aagccatctc ccagggccac caggccttct 361 tattggaggg ggacagcagc tccagggatg tcttctcttg caatgtgggt aacctccaac 421 ctgggtcgaa ggcggcagtc accctgaagt atgtgcagga gctgcctctg gaagcagatg 481 gggctctgcg ctttgtgctc ccagctgtcc tgaatcctag ataccagttc tctgggtcgt 541 ctaaggacag ttgccttaat gtgaagactc ctatagtccc tgtggaggac ctgccctaca 601 cactcagcat ggtcgccacc atagattccc agcatggcat tgagaaggtc caatccaact 661 gccccttgag tcctaccgag tacctaggag aggacaagac ttctgctcag gtttccctgg 721 ctgctggaca caagtttgat cgggacgtgg aactcctgat ttactacaat gaggtgcata 781 cccccagcgt ggttttggag atggggatgc ctaacatgaa gccaggtcat ttgatgggag 841 atccatctgc aatggtgagt ttctatccaa atatcccaga agatcaacca tcaaatacct 901 gtggagagtt tatctttctc atggaccgct cgggaagtat gcagagcccc atgagtagcc 961 aggatacatc TCAGCTGCGA ATACAGGCAG CCAAGGAAAC ACTGATTTTG CTGCTGAAGA 1021 GTTTACCTAT AGGCTGTTAT TTCAACATCT ATGGATTTGG CTCTTCCTAT GAGGCATGCT 1081 TTCCGGAGAG TGTGAAGTAC ACTCAGCAAA CAATGGAGGA GGCTCTGGGG AGAGTGAAGC 1141 TTATGCAGGC CGACCTAGGG GGCACTGAAA TCTTGGCACC ACTCCAGAAC ATTTACAGGG 1201 GACCCTCCAT CCCAGGCCAC CCCCTACAGC TTTTTGTCTT TACAGATGGA GAAGTTACAG 1261 ACACGTTTAG TGTAATTAAA GAAGTTAGGA TCAACAGACA GAAACACAGG TACCCAACTT 1321 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1381 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1441 CTTGTGGAAA GGACGAAATC GGGACTGATG GGCAGCATGG ACGCGTTAAG TCgacaatca 1501 acctctggat tacaaaattt gtgaaagatt