Construct: ORF TRCN0000465538

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003223.1_s317c1
Derived from:
ccsbBroadEn_00947
DNA Barcode:
AATCGGGACTGATGGGCAGCATGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VWA5A (4013)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465538
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4013 VWA5A von Willebrand factor A dom... NM_198315.2 100% 100%
2 human 4013 VWA5A von Willebrand factor A dom... NM_001130142.2 52.7% 52.7% 1246_2358del
3 human 4013 VWA5A von Willebrand factor A dom... NM_014622.4 52.7% 52.7% 1246_2358del
4 human 4013 VWA5A von Willebrand factor A dom... XM_011542828.2 51.7% 51.7% 1_48del;1294_2406del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1311
ORF length:
1245
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gcacttctgt ggcctactca ccctccaccg ggagccagtg ccgctgaaga
121gtatctctgt gagcgtgaac atttacgagt ttgtggctgg tgtgtctgca actttgaact
181acgagaatga ggagaaagtt cctttggagg ccttctttgt gttccccatg gatgaagact
241ctgctgttta cagctttgag gccttggtgg atgggaagaa aattgtagca gaattacaag
301acaagatgaa ggcccgcacc aactatgaga aagccatctc ccagggccac caggccttct
361tattggaggg ggacagcagc tccagggatg tcttctcttg caatgtgggt aacctccaac
421ctgggtcgaa ggcggcagtc accctgaagt atgtgcagga gctgcctctg gaagcagatg
481gggctctgcg ctttgtgctc ccagctgtcc tgaatcctag ataccagttc tctgggtcgt
541ctaaggacag ttgccttaat gtgaagactc ctatagtccc tgtggaggac ctgccctaca
601cactcagcat ggtcgccacc atagattccc agcatggcat tgagaaggtc caatccaact
661gccccttgag tcctaccgag tacctaggag aggacaagac ttctgctcag gtttccctgg
721ctgctggaca caagtttgat cgggacgtgg aactcctgat ttactacaat gaggtgcata
781cccccagcgt ggttttggag atggggatgc ctaacatgaa gccaggtcat ttgatgggag
841atccatctgc aatggtgagt ttctatccaa atatcccaga agatcaacca tcaaatacct
901gtggagagtt tatctttctc atggaccgct cgggaagtat gcagagcccc atgagtagcc
961aggatacatc TCAGCTGCGA ATACAGGCAG CCAAGGAAAC ACTGATTTTG CTGCTGAAGA
1021GTTTACCTAT AGGCTGTTAT TTCAACATCT ATGGATTTGG CTCTTCCTAT GAGGCATGCT
1081TTCCGGAGAG TGTGAAGTAC ACTCAGCAAA CAATGGAGGA GGCTCTGGGG AGAGTGAAGC
1141TTATGCAGGC CGACCTAGGG GGCACTGAAA TCTTGGCACC ACTCCAGAAC ATTTACAGGG
1201GACCCTCCAT CCCAGGCCAC CCCCTACAGC TTTTTGTCTT TACAGATGGA GAAGTTACAG
1261ACACGTTTAG TGTAATTAAA GAAGTTAGGA TCAACAGACA GAAACACAGG TACCCAACTT
1321TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1381CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1441CTTGTGGAAA GGACGAAATC GGGACTGATG GGCAGCATGG ACGCGTTAAG TCgacaatca
1501acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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