Construct: ORF TRCN0000465548
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016495.1_s317c1
- Derived from:
- ccsbBroadEn_03953
- DNA Barcode:
- GGTCATCTCACATTTCGTTTGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS25 (64432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465548
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64432 | MRPS25 | mitochondrial ribosomal pro... | NM_022497.5 | 100% | 100% | |
| 2 | human | 64432 | MRPS25 | mitochondrial ribosomal pro... | NR_135247.2 | 12.5% | 1_121del;641_4148del | |
| 3 | human | 64432 | MRPS25 | mitochondrial ribosomal pro... | NR_135246.2 | 12.2% | 1_121del;451_539del;730_4237del | |
| 4 | human | 64432 | MRPS25 | mitochondrial ribosomal pro... | XR_001740234.2 | 6.1% | 1_110del;630_8420del | |
| 5 | mouse | 64658 | Mrps25 | mitochondrial ribosomal pro... | NM_025578.4 | 85% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 585
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc catgaagggc cgcttcccca tccgccgcac cctgcaatat ctgagccagg 121 ggaacgtggt gttcaaggac tccgtgaagg tcatgacagt gaattacaac acgcatgggg 181 agctgggcga gggcgccagg aagtttgtgt ttttcaacat accTCAGATT CAATACAAAA 241 ACCCTTGGGT GCAGATCATG ATGTTTAAGA ACATGACGCC GTCACCCTTC CTGCGATTCT 301 ACTTAGATTC TGGGGAGCAG GTCCTGGTGG ATGTGGAGAC CAAGAGCAAT AAGGAGATCA 361 TGGAGCACAT CAGAAAAATC TTGGGGAAGA ATGAGGAAAC CCTCAGGGAA GAGGAGGAGG 421 AGAAAAAGCA GCTTTCTCAC CCAGCCAACT TCGGCCCTCG AAAGTACTGC CTGCGGGAGT 481 GCATCTGTGA AGTGGAAGGG CAGGTGCCCT GCCCCAGCCT GGTGCCATTA CCCAAGGAGA 541 TGAGGGGGAA GTACAAAGCC GCTCTGAAAG CCGATGCCCA GGACTACCCA ACTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA GGTCATCTCA CATTTCGTTT GACGACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt