Construct: ORF TRCN0000465551
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010555.1_s317c1
- Derived from:
- ccsbBroadEn_00992
- DNA Barcode:
- CCTATCATCCAGACGTTTAAGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MDK (4192)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465551
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4192 | MDK | midkine | NM_001012333.2 | 100% | 100% | |
| 2 | human | 4192 | MDK | midkine | NM_001012334.2 | 100% | 100% | |
| 3 | human | 4192 | MDK | midkine | NM_001270550.1 | 100% | 100% | |
| 4 | human | 4192 | MDK | midkine | NM_001270551.2 | 100% | 100% | |
| 5 | human | 4192 | MDK | midkine | NM_002391.5 | 100% | 100% | |
| 6 | human | 4192 | MDK | midkine | XM_011520116.2 | 100% | 100% | |
| 7 | human | 4192 | MDK | midkine | XM_017017764.1 | 100% | 100% | |
| 8 | human | 4192 | MDK | midkine | NM_001270552.2 | 60.8% | 60.8% | 74_75ins168 |
| 9 | human | 4192 | MDK | midkine | NR_073039.2 | 49.6% | (many diffs) | |
| 10 | mouse | 17242 | Mdk | midkine | NM_001012335.2 | 87.6% | 86% | (many diffs) |
| 11 | mouse | 17242 | Mdk | midkine | NM_001012336.2 | 87.6% | 86% | (many diffs) |
| 12 | mouse | 17242 | Mdk | midkine | NM_001291481.1 | 87.6% | 86% | (many diffs) |
| 13 | mouse | 17242 | Mdk | midkine | NM_010784.5 | 87.6% | 86% | (many diffs) |
| 14 | mouse | 17242 | Mdk | midkine | NM_001291482.1 | 56.8% | 55.9% | (many diffs) |
| 15 | mouse | 17242 | Mdk | midkine | XM_011239335.2 | 56.8% | 55.9% | (many diffs) |
| 16 | mouse | 17242 | Mdk | midkine | NM_001291483.1 | 51.9% | 52.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 495
- ORF length:
- 429
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gcaccgaggc ttcctcctcc tcaccctcct cgccctgctg gcgctcaccT 121 CCGCGGTCGC CAAAAAGAAA GATAAGGTGA AGAAGGGCGG CCCGGGGAGC GAGTGCGCTG 181 AGTGGGCCTG GGGGCCCTGC ACCCCCAGCA GCAAGGATTG CGGCGTGGGT TTCCGCGAGG 241 GCACCTGCGG GGCCCAGACC CAGCGCATCC GGTGCAGGGT GCCCTGCAAC TGGAAGAAGG 301 AGTTTGGAGC CGACTGCAAG TACAAGTTTG AGAACTGGGG TGCGTGTGAT GGGGGCACAG 361 GCACCAAAGT CCGCCAAGGC ACCCTGAAGA AGGCGCGCTA CAATGCTCAG TGCCAGGAGA 421 CCATCCGCGT CACCAAGCCC TGCACCCCCA AGACCAAAGC AAAGGCCAAA GCCAAGAAAG 481 GGAAGGGAAA GGACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 541 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 601 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCTATCATCC AGACGTTTAA 661 GGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt