Construct: ORF TRCN0000465567
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015785.1_s317c1
- Derived from:
- ccsbBroadEn_08906
- DNA Barcode:
- CTAAAACCATGTCTCAATATCTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NUP37 (79023)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465567
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79023 | NUP37 | nucleoporin 37 | NM_024057.4 | 99.8% | 98.4% | 966G>A |
| 2 | human | 79023 | NUP37 | nucleoporin 37 | XR_944712.2 | 58.2% | (many diffs) | |
| 3 | mouse | 69736 | Nup37 | nucleoporin 37 | NM_027191.2 | 87.7% | 89.8% | (many diffs) |
| 4 | mouse | 69736 | Nup37 | nucleoporin 37 | NM_028334.4 | 87.7% | 89.8% | (many diffs) |
| 5 | mouse | 69736 | Nup37 | nucleoporin 37 | XM_006514057.2 | 71.9% | 73.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1029
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcaagatgcc tcaagaaatg ctgcctacac tgtggattgt gaagattatg 121 tgcatgtggt agaatttaat ccctttgaga atggggattc aggaaaccta attgcatatg 181 gtggcaataa ttatgtggtc attggcacgt gtacgtttca ggaagaagaa gcagacgttg 241 aaggcattca gtataaaaca cttcgaacat ttcaccatgg agtcagggtt gatggcatag 301 cttggagccc agagactaga cttgattcat tgcctccagt aatcaaattt tgtacttcag 361 ctgctgatat gaaaattaga ttatttactt cagatcttca ggataaaaat gaatataagg 421 ttttagaggg ccataccgat ttcattaatg gtttggtgtt tgatcccaaa gaaggccaag 481 aaattgcaag tgtgagtgac gatcacacct gcaggatttg gaacttggaa ggagtgcaaa 541 cagctcattt tgttcttcat tctcctggca tgagtgtgtg ctggcatcct gaggagactt 601 ttaagctaat ggttgcagag aagaatggaa caatccggtt ttatgatctt ttggcccaac 661 aggctatttt atctcttgaa tcagaacaag tgccattaat gtcagcacac tggtgcttaa 721 aaaacacctt caaagttgGA GCCGTTGCAG GAAATGATTG GTTAATTTGG GATATTACTC 781 GGTCCAGTTA TCCTCAAAAT AAGAGACCTG TTCACATGGA TCGAGCCTGC TTATTCAGGT 841 GGTCCACAAT TAGTGAAAAT CTGTTTGCAA CCACTGGTTA TCCTGGCAAA ATGGCAAGCC 901 AGTTTCAAAT TCATCATTTA GGACACCCTC AGCCCATCCT CATGGGTTCT GTAGCCGTTG 961 GATCTGGACT GTCCTGGCAT CGAACTCTCC CTCTGTGTGT AATTGGAGGA GACCACAAGC 1021 TGTTGTTTTG AGTGACTGAA GTATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TAAAACCATG 1201 TCTCAATATC TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att