Construct: ORF TRCN0000465570
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000037.1_s317c1
- Derived from:
- BRDN0000391615
- DNA Barcode:
- AAGTGGGAGAGTAAGTGCCTGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRB4 (5545)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465570
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5545 | PRB4 | proline rich protein BstNI ... | NM_001261399.2 | 55.9% | 55.6% | 146_379del;418G>C |
| 2 | human | 5545 | PRB4 | proline rich protein BstNI ... | NM_002723.6 | 40.3% | 40% | 146_586del;625G>C |
| 3 | human | 5544 | PRB3 | proline rich protein BstNI ... | NM_006249.5 | 27.1% | 24.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 369
- ORF length:
- 300
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgctgatt ctgctgtcag tggccctgct ggccctgagc TCAGCTGAGA 121 GTTCAAGTGA AGATGTCAGC CAGGAAGAAT CTCTCTTCCT AATATCAGGA AAGCCAGAAG 181 GACGACGCCC ACAAGGAGGA AACCAGCCCC AACGTCCCCC ACCTCCTGGA AAGCCACAAG 241 GCCCACCCCC ACCAGGAGGC AATCCCCAGC AGCCTCAGGC ACCTCCTGCT GGAAAGCCCC 301 AGGGGCCACC TCCACCTCCT CAAGGGGGCA GGCCACCCAG ACCTGCCCAG GGACAACAGC 361 CTCCCCAGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGTGG GAGAGTAAGT GCCTGGTCAC 541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt