Construct: ORF TRCN0000465571
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011709.1_s317c1
- Derived from:
- ccsbBroadEn_03017
- DNA Barcode:
- TGTAGCCATAGAGGTGAGCAAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RND1 (27289)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465571
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27289 | RND1 | Rho family GTPase 1 | NM_014470.4 | 100% | 100% | |
| 2 | mouse | 223881 | Rnd1 | Rho family GTPase 1 | NM_172612.3 | 89.5% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 762
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggagagacgg gccccccagc cagtcgtggc cagatgtaag ctcgttctgg 121 tcggggacgt gcagtgtggg aagaccgcga tgttgcaagt gttagcgaag gattgctatc 181 cagagaccta tgtgcccacc gtgttcgaaa attacacagc ctgtttggag acagaggaac 241 agagggtgga gcttagtctc tgggatacct caggatctcc ctactacgat aatgtccgtc 301 cactctgcta cagcgactcg gatgcagtat tactatgttt tgacatcagc cgtccagaga 361 cagtggacag cgcactcaag aagtggagga cagaaatcct agattattgt cccagcaccc 421 gcgttttgct cattggctgc aagacagacc tgcgaacaga cctgagtact ctgatggagc 481 tgtcccacca gaagcaggcg cccatctccT ATGAGCAGGG TTGTGCAATA GCAAAGCAGC 541 TGGGTGCAGA AATCTACCTG GAAGGCTCAG CTTTCACCTC AGAAAAGAGC ATCCACAGCA 601 TCTTTCGGAC GGCATCCATG CTGTGTCTGA ACAAGCCTAG CCCACTGCCC CAGAAGAGCC 661 CTGTCCGAAG CCTCTCCAAA CGACTGCTCC ACCTCCCCAG TCGCTCTGAA CTCATCTCTT 721 CTACCTTCAA GAAGGAAAAG GCCAAAAGCT GTTCCATTAT GTGCCCAACT TTCTTGTACA 781 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 841 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 901 AGGACGATGT AGCCATAGAG GTGAGCAAAG GACGCGTTAA GTCgacaatc aacctctgga 961 ttacaaaatt tgtgaaagat t