Construct: ORF TRCN0000465578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014091.1_s317c1
- Derived from:
- ccsbBroadEn_02534
- DNA Barcode:
- TGCATGACACCAAGGCCATCATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UTP14A (10813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10813 | UTP14A | UTP14A small subunit proces... | NM_006649.4 | 100% | 100% | |
| 2 | human | 9724 | UTP14C | UTP14C small subunit proces... | NM_021645.6 | 93.7% | 89.6% | (many diffs) |
| 3 | human | 10813 | UTP14A | UTP14A small subunit proces... | NM_001166221.1 | 93.2% | 93.2% | 380_381ins156 |
| 4 | human | 10813 | UTP14A | UTP14A small subunit proces... | XM_017029237.1 | 78.1% | 76% | (many diffs) |
| 5 | human | 10813 | UTP14A | UTP14A small subunit proces... | XM_017029238.2 | 58.2% | 58.2% | 0_1ins966 |
| 6 | human | 440138 | ALG11 | ALG11 alpha-1,2-mannosyltra... | NR_036571.3 | 40.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2379
- ORF length:
- 2313
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgcgaaccgg cttgcagaga gccttctggc tttgagccaa caggaagaac 121 tagcggattt gccaaaagac tacctcttga gtgagagtga agatgagggg gacaatgatg 181 gagagagaaa gcatcaaaag cttctggaag caatcagttc ccttgatgga aagaataggc 241 ggaaattggc tgagaggtct gaggctagtc tgaaggtgtc agagttcaat gtcagttctg 301 aaggatcagg agaaaagctg gtccttgcag atctgcttga gcctgttaaa acttcatctt 361 ctttggccac tgtgaaaaag caactgagta gagtcaaatc aaagaagaca gtggagttac 421 ctctgaacaa agaagagatt gaacggatcc acagagaagt agcattcaat aaaaccgcac 481 aagtcctctc caaatgggac cctgtcgtcc tgaagaaccg gcaggcagag cagctggttt 541 ttcccctgga gaaagaggag ccagccattg ctcccattga acatgtgctc agtggctgga 601 aggcaagaac tcccctggag caggaaattt tcaacctcct ccataagaac aagcagccag 661 tgacagaccc tttactgacc cctgtggaaa aggcctctct ccgagccatg agcctagaag 721 aggcaaagat gcgacgagca gagcttcaga gggctcgggc tctgcagtcc tactatgagg 781 ccaaggctcg aagagagaag aaaatcaaaa gtaaaaagta tcacaaagtc gtgaagaaag 841 gaaaggccaa gaaagcccta aaagagtttg agcagctgcg gaaggttaat ccagctgcag 901 cactagaaga actggaaaaa attgaaaagg ccagaatgat ggaaagaatg agccttaagc 961 accaaaacag tgggaaatgg gccaagtcaa aggcaattat ggccaaatat gacctggagg 1021 ctcgccaagc tatgcaggaa cagttgtcta agaacaaaga actgacacag aaactccagg 1081 tagcctctga gagtgaggaa gaggagggag gcacagaaga tgtggaagaa ctccttgtcc 1141 ctgatgtagt gaatgaagtg cagatgaatg cagatgggcc gaatccctgg atgctcagga 1201 gctgcaccag tgacaccaaa gaggctgcaa cccaggagga ccctgagcaa ctgccagagc 1261 ttgaggccca tggagtttct gaaagtgagg gagaagaaag accagtggca gaagaagaaa 1321 ttttgttgag agaatttgag gaaaggcgat cccttagaaa aagatctgag ctcagccaag 1381 atgctgagcc agcaggcagt caagaaacaa aagattctgg cagccaggag gtgctgtctg 1441 aattgagagt actatctcag aaattgaagg aaaaccatca gtccaggaag caaaaagcaa 1501 gttcagaggg gactattccc caggtccaga gagaggaacc tgccccagaa gaagaggagc 1561 ccctgttgct acagagacca gagagagtac agacgctgga agagctagaa gagctgggaa 1621 aagaagaatg ttttcaaaat aaggagcttc ccagacctgt gttagaaggg cagcagtcag 1681 agaggacccc aaataatcgc cctgatgccc ctaaggagaa gaaaaagaag gagcaaatga 1741 tcgacctaca gaacctccta accacacaat ctccctccgt gaagtctttg gcagttccca 1801 caatagagga gctggaagat gaagaggaga gaaaccatag gcagatgata aaggaagctt 1861 ttgctgggga tgatgtcatc agagatttct tgaaagagaa gagggaagct gtggaggcga 1921 gtaagccaaa ggacgtggac ctgacactac ctggctgggg cgagtggggt ggtgtgggcc 1981 taaagcccag tgccaagaaa agacgccggt ttctcattaa agcccctgag ggTCCTCCAA 2041 GAAAAGATAA GAATTTGCCA AATGTGATTA TCAATGAGAA GCGCAACATC CACGCAGCTG 2101 CTCATCAGGT ACGAGTGCTT CCATATCCAT TTACCCACCA TTGGCAATTT GAAAGGACCA 2161 TCCAGACCCC CATAGGATCC ACATGGAACA CCCAGAGGGC TTTCCAAAAG CTGACTACTC 2221 CCAAGGTCGT CACCAAGCCA GGCCATATCA TTAACCCCAT AAAAGCAGAA GACGTGGGCT 2281 ACCGGTCTTC CTCAAGGTCG GACCTGTCTG TCATACAGAG GAATCCAAAA CGAATCACCA 2341 CACGTCACAA AAAACAGCTG AAGAAATGCT CTGTAGATTC CCCAACTTTC TTGTACAAAG 2401 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2461 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2521 ACGATGCATG ACACCAAGGC CATCATCGAC GCGTTAAGTC gacaatcaac ctctggatta 2581 caaaatttgt gaaagatt