Construct: ORF TRCN0000465583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003366.1_s317c1
- Derived from:
- ccsbBroadEn_09225
- DNA Barcode:
- AGTTCATCCGGCCACCCTCAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BUD13 (84811)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465583
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84811 | BUD13 | BUD13 homolog | NM_032725.4 | 99.9% | 100% | 480G>A |
| 2 | human | 84811 | BUD13 | BUD13 homolog | XM_011543035.2 | 93.6% | 92.8% | (many diffs) |
| 3 | human | 84811 | BUD13 | BUD13 homolog | NM_001159736.1 | 78.2% | 78.3% | 480G>A;632_633ins402 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1923
- ORF length:
- 1857
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagctccg ccgctttcca aggccgagta tctgaagcgt tacttgtccg 121 gggcagatgc cggcgtcgac cggggatctg agtccggtcg caagcgtcgc aaaaagcggc 181 cgaagcctgg cggggccggc ggcaagggaa tgcggattgt ggatgatgat gtgagctgga 241 cagctatctc cacaaccaaa ctagaaaagg aggaagagga agatgatgga gatttgcctg 301 tggtggcaga gtttgtggat gagcggccag aagaggtaaa gcagatggag gcctttcgtt 361 ccagtgccaa atggaagctt ctgggaggcc acaacgaaga cctaccctca aacagacatt 421 ttcgtcacga taccccggat tcatctccta ggagggtccg tcatggtacc ccagatccat 481 ctcctaggaa ggaccgtcat gacaccccgg atccatctcc taggagggcc cgtcatgaca 541 ccccagatcc ttctcccctc agaggggctc gtcatgactc agacacatct cctcccagga 601 ggatccgtca tgactcctca gacacttcac ccccaaggag ggcccgtcat gattctccag 661 atccttctcc cccaaggagg cctcagcata attcttcagg tgcatctcct aggagagtcc 721 gtcatgattc accagatccc tctcctccta ggcgagcccg tcatggttcc tcagatatct 781 cttcccccag aagggtccat aacaactccc ctgacacatc taggaggact cttggctctt 841 cagacacaca gcaactcaga agggcccgtc atgactcccc tgatttggct cctaatgtca 901 cttattccct gcccagaacc aaaagtggta aagccccaga aagagcctct agcaagactt 961 ctccacattg gaaggagtca ggagcctccc atttgtcatt cccaaagaac agcaaatatg 1021 agtatgaccc tgacatctct cctccacgaa aaaagcaagc aaaatcccat tttggagaca 1081 agaagcagct tgattccaaa ggtgactgcc agaaagcaac tgattcagac ctttcttctc 1141 cacggcataa acaaagtcca gggcaccagg attctgattc agatctgtca cctccacgga 1201 atagacctag acaccggagc tctgattctg acctctctcc accaaggagg agacagagga 1261 ccaaatcttc tgattctgac ctgtccccgc ctcgaaggag tcagcctcct ggaaagaagg 1321 ctgcacacat gtattctggg gctaaaactg ggttggtgtt aactgacata cagcgagaac 1381 agcaggagct caaggaacag gatcaagaaa ccatggcatt tgaagctgaa tttcaatatg 1441 ctgaaaccgt atttcgagat aagtctggtc gtaagaggaa tttgaaactc gaacgtttag 1501 agcaaaggag gaaagcagaa aaggactcag agagagatga gctgtatgcc cagtgGGGAA 1561 AAGGGCTTGC CCAGAGCCGG CAACAGCAAC AAAATGTGGA GGATGCAATG AAAGAGATGC 1621 AAAAGCCTCT GGCCCGCTAT ATTGATGACG AAGATCTGGA TAGGATGCTA AGAGAACAGG 1681 AAAGAGAGGG GGACCCTATG GCCAACTTCA TCAAGAAGAA TAAGGCCAAG GAGAACAAGA 1741 ATAAAAAAGT GAGACCTCGC TACAGTGGTC CAGCACCTCC TCCCAACAGA TTTAATATCT 1801 GGCCTGGATA TCGCTGGGAC GGAGTGGACA GATCCAATGG ATTTGAACAG AAGCGCTTTG 1861 CCAGGCTTGC CAGCAAGAAG GCAGTGGAGG AACTTGCCTA CAAATGGAGT GTTGAGGATA 1921 TGTACCCAAC TTACTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGAAG TTCATCCGGC CACCCTCAAC CAACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt