Construct: ORF TRCN0000465626
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012262.1_s317c1
- Derived from:
- ccsbBroadEn_07000
- DNA Barcode:
- CTGGGTGGGATTCAGCACTTATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RO60 (6738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465626
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6738 | RO60 | Ro60, Y RNA binding protein | NM_001173524.2 | 99.9% | 100% | 15A>G |
| 2 | human | 6738 | RO60 | Ro60, Y RNA binding protein | NM_004600.5 | 99.9% | 100% | 15A>G |
| 3 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_006711497.3 | 99.9% | 100% | 15A>G |
| 4 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_017002181.2 | 99.9% | 100% | 15A>G |
| 5 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_017002182.1 | 99.9% | 100% | 15A>G |
| 6 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_024449332.1 | 99.9% | 100% | 15A>G |
| 7 | human | 6738 | RO60 | Ro60, Y RNA binding protein | NM_001042370.2 | 98.4% | 97.9% | (many diffs) |
| 8 | human | 6738 | RO60 | Ro60, Y RNA binding protein | NM_001042369.2 | 96.9% | 95.1% | (many diffs) |
| 9 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_006711495.4 | 96% | 95.8% | 15A>G;581_646del |
| 10 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_006711496.3 | 96% | 95.8% | 15A>G;581_646del |
| 11 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_017002180.1 | 96% | 95.8% | 15A>G;581_646del |
| 12 | human | 6738 | RO60 | Ro60, Y RNA binding protein | NM_001173525.1 | 95.8% | 95.5% | (many diffs) |
| 13 | human | 6738 | RO60 | Ro60, Y RNA binding protein | NM_001331020.2 | 48.8% | 48.8% | 0_1ins825 |
| 14 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_011509922.2 | 48.8% | 48.8% | 0_1ins825 |
| 15 | human | 6738 | RO60 | Ro60, Y RNA binding protein | XM_017002183.1 | 48.8% | 48.8% | 0_1ins825 |
| 16 | mouse | 20822 | Trove2 | TROVE domain family, member 2 | NM_013835.2 | 85.3% | 89.7% | (many diffs) |
| 17 | mouse | 20822 | Trove2 | TROVE domain family, member 2 | XM_017319451.1 | 85.3% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1680
- ORF length:
- 1614
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaatctgtg aaccaaatgc agccactgaa tgagaagcag atagccaatt 121 ctcaggatgg atatgtatgg caagtcactg acatgaatcg actacaccgg ttcttatgtt 181 tcggttctga aggtgggact tattatatca aagaacagaa gttgggcctt gaaaatgctg 241 aagctttaat tagattgatt gaagatggca gaggatgtga agtgatacaa gaaataaagt 301 catttagtca agaaggcaga accacaaagc aagagcctat gctctttgca cttgccattt 361 gttcccagtg ctccgacata agcacaaaac aagcagcatt taaagctgtt tctgaagttt 421 gtcgcattcc tacccatctc tttactttta tccagtttaa gaaagatctg aaggaaagca 481 tgaaatgtgg catgtggggt cgtgccctcc ggaaggctat agcggactgg tacaatgaga 541 aaggtggcat ggcccttgct ctggcagtta caaaatataa acagagaaat ggctggtctc 601 acaaagatct attaagattg tcacatctta aaccttccag tgaaggactt gcaattgtga 661 ccaaatatat tacaaagggc tggaaagaag ttcatgaatt gtataaagaa aaagcactct 721 ctgtggagac tgaaaaatta ttaaagtatc tggaggctgt agagaaagtg aagcgcacaa 781 gagatgagct agaagtcatt catctaatag aagaacatag attagttaga gaacatcttt 841 taacaaatca cttaaagtct aaagaggtat ggaaggcttt gttacaagaa atgccgctta 901 ctgcattact aaggaatcta ggaaagatga ctgctaattc agtacttgaa ccaggaaatt 961 cagaagtatc tttagtatgt gaaaaactgt gtaatgaaaa actattaaaa aaggctcgta 1021 tacatccatt tcatattttg atcgcattag aaacttacaa gacaggtcat ggtctcagag 1081 ggaaactgaa gtggcgccct gatgaagaaa ttttgaaagc attggatgct gctttttata 1141 aaacatttaa gacagttgaa ccaactggaa aacgtttctt actagctgtt gatgtcagtg 1201 cttctatgaa ccaaagagtt ttgggtagta tactcaacgc tagtacagtt gctgcagcaa 1261 tgtgcatggt tgtcacacga acagaaaaag attcttatGT AGTTGCTTTT TCCGATGAAA 1321 TGGTACCATG TCCAGTGACT ACAGATATGA CCTTACAACA GGTTTTAATG GCTATGAGTC 1381 AGATCCCAGC AGGTGGAACT GATTGCTCTC TTCCAATGAT CTGGGCTCAG AAGACAAACA 1441 CACCTGCTGA TGTCTTCATT GTATTCACTG ATAATGAGAC CTTTGCTGGA GGTGTCCATC 1501 CTGCTATTGC TCTGAGGGAG TATCGAAAGA AAATGGATAT TCCAGCTAAA TTGATTGTTT 1561 GTGGAATGAC ATCAAATGGT TTCACCATTG CAGACCCAGA TGATAGAGGC ATGTTGGATA 1621 TGTGCGGCTT TGATACTGGA GCTCTGGATG TAATTCGAAA TTTCACATTA GATATGATTT 1681 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1741 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1801 TTTATATATC TTGTGGAAAG GACGACTGGG TGGGATTCAG CACTTATCTA CGCGTTAAGT 1861 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt