Construct: ORF TRCN0000465632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005828.1_s317c1
- Derived from:
- ccsbBroadEn_12496
- DNA Barcode:
- GATACCCTCCTCTGCCCGTTCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX31 (64794)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64794 | DDX31 | DEAD-box helicase 31 | NM_001322342.1 | 99.9% | 99.8% | 1864A>G |
| 2 | human | 64794 | DDX31 | DEAD-box helicase 31 | NM_001322343.1 | 93.3% | 93.2% | 1_144del;2008A>G |
| 3 | human | 64794 | DDX31 | DEAD-box helicase 31 | NM_001322341.2 | 89.2% | 89.1% | 1_243del;2107A>G |
| 4 | human | 64794 | DDX31 | DEAD-box helicase 31 | XM_005272207.4 | 89.2% | 89.1% | 1_243del;2107A>G |
| 5 | human | 64794 | DDX31 | DEAD-box helicase 31 | NM_022779.8 | 79.1% | 79% | 1_531del;2395A>G |
| 6 | human | 64794 | DDX31 | DEAD-box helicase 31 | NM_001322340.1 | 70.5% | 67% | (many diffs) |
| 7 | human | 64794 | DDX31 | DEAD-box helicase 31 | XM_011518921.3 | 70.5% | 70% | (many diffs) |
| 8 | human | 64794 | DDX31 | DEAD-box helicase 31 | XM_006717236.4 | 60.3% | 55.6% | 1_531del;1946_1947ins194;2073_2074ins286 |
| 9 | human | 64794 | DDX31 | DEAD-box helicase 31 | XM_011518922.3 | 60.3% | 55.6% | 1_531del;1946_1947ins194;2073_2074ins286 |
| 10 | human | 64794 | DDX31 | DEAD-box helicase 31 | NM_138620.1 | 47.9% | 47.9% | 1_531del;1755_1756ins798 |
| 11 | human | 64794 | DDX31 | DEAD-box helicase 31 | NR_136309.2 | 45.2% | (many diffs) | |
| 12 | human | 64794 | DDX31 | DEAD-box helicase 31 | XR_246600.5 | 43.6% | (many diffs) | |
| 13 | human | 64794 | DDX31 | DEAD-box helicase 31 | XR_929836.3 | 42.3% | (many diffs) | |
| 14 | human | 64794 | DDX31 | DEAD-box helicase 31 | XR_001746366.2 | 41.8% | (many diffs) | |
| 15 | human | 64794 | DDX31 | DEAD-box helicase 31 | NM_001322344.1 | 16.1% | 15.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2088
- ORF length:
- 2022
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ttctccaaag aagcattcgg ttagcacaag tgatagaaac caggaggaga 121 gacagtgcat taagacttca tcactgttta aaaacaaccc tgacattcca gaactccaca 181 gacctgtggt aaagcaggtg caagaaaaag tgtttacttc agctgctttt catgagctgg 241 gcctccaccc acatttaatt tccacaataa atacggtctt aaaaatgtct agtatgacca 301 gtgttcagaa gcaaagtatt cctgtgttgc tggaaggcag agatgctctc gtgagatccc 361 agacgggctc aggtaaaact cttgcctatt gcatccctgt ggtccagtcc cttcaagcaa 421 tggagtcaaa aatacagcgc agtgatggcc cctatgccct ggtgctcgtg ccaacgagag 481 agctagctct acaaagcttt gacactgtcc agaaactgct taagcctttc acctggattg 541 tgcctggagt gttaatggga ggagagaaga gaaaatcaga aaaggccaga ctccgcaaag 601 gaataaatat ccttatctca actcctggac gcctggtgga tcatataaaa tccacaaaga 661 acattcattt tagtcggctg cggtggttgg tgtttgatga agcagacaga atcttggatt 721 tgggttttga aaaggacatc acagtgatac ttaatgctgt aaatgctgaa tgccaaaaac 781 gacagaatgt cttgctatca gcgacactca cagaaggtgt aacgcggcta gctgatatca 841 gtttgcatga tccagtcagt atttctgtcc tggacaagag ccatgaccag ttgaacccaa 901 aggacaaagc ggtccaggag gtctgtcctc caccagctgg cgacaagctg gacagctttg 961 caataccaga gagtctcaag cagcatgtga ctgtggttcc cagcaaactg aggcttgtct 1021 gcctagcggc cttcatcctt cagaaatgca agtttgagga agaccagaag atggttgtct 1081 ttttctcaag ttgcgagctg gtggagttcc actacagcct cttcctacag accctgctga 1141 gcagctcagg ggcgccggca tcagggcagt tgccatctgc ctccatgcga ttaaaattcc 1201 tacggctgca tggcggcatg gagcaggagg aaagaacagc agtgtttcag gaattttcac 1261 attccagaag aggcgtcctt ctttgcacgg atgttgcagc tcggggctta gatctccctc 1321 aagtcacgtg gattgttcag tacaacgctc catcttcacc tgcagaatac atccaccgga 1381 ttggaagaac cgcccggatt ggctgccatg ggagcagcct gctcattttg gctccttcgg 1441 aggcagaata tgtcaactcg ttggcttctc acaaaatcaa cgtttctgag attaagatgg 1501 aagatatttt gtgtgttctg acaagagatg attgttttaa agggaaacga tggggagccc 1561 agaaatccca tgctgttggc ccccaggaaa tccgagagcg agccacagtc ttgcagacgg 1621 tatttgaaga ttacgtgcac tccagtgaga ggagggtctc ctgggcaaag aaagctctgc 1681 agtccttcat ccaagcctac gccacctacc ccagggagct gaagcacaTC TTCCACGTCC 1741 GATCCCTCCA CCTTGGGCAT GTGGCGAAGA GCTTCGGACT AAGAGATGCC CCCAGGAATC 1801 TTAGTGCCTT GACTAGAAAG AAGAGGAAAG CACACGTGAA AAGGCCTGAC CTTCATAAGA 1861 AGACCCAGAG TAAACACAGC CTCGCTGAAA TCCTACGTTC GGAATACTCA AGCGGCATGG 1921 AGGCCGACGT CGCCAAGGTC AAAAAGCAAA ACGCACCTGG AGAGCCTGGT GGCCGGCCCC 1981 TGCAGCACAG TCTGCAGCCG ACACCCTGCT TTGGCCGTGG GAAAACATTA AAATGGAGAA 2041 AAACCCAAAA AGGTGTACAG CGGGACAGCA AGACTTCCCA GAAAGTTTAC CCAACTTTCT 2101 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2161 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2221 GTGGAAAGGA CGAGATACCC TCCTCTGCCC GTTCCACACG CGTTAAGTCg acaatcaacc 2281 tctggattac aaaatttgtg aaagatt