Transcript: Human NM_022779.8

Homo sapiens DEAD-box helicase 31 (DDX31), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
DDX31 (64794)
Length:
4577
CDS:
153..2708

Additional Resources:

NCBI RefSeq record:
NM_022779.8
NBCI Gene record:
DDX31 (64794)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_022779.8, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232438 ATCTGCCTCCATGCGATTAAA pLKO_005 1793 CDS 100% 15.000 21.000 N DDX31 n/a
2 TRCN0000050372 CGGTTAGCACAAGTGATAGAA pLKO.1 706 CDS 100% 5.625 7.875 N DDX31 n/a
3 TRCN0000050369 GCAGAATACATCCACCGGATT pLKO.1 1980 CDS 100% 4.050 5.670 N DDX31 n/a
4 TRCN0000050371 GCTGAAATCCTACGTTCGGAA pLKO.1 2502 CDS 100% 2.640 3.696 N DDX31 n/a
5 TRCN0000232436 GACGCCTGGTGGATCATATAA pLKO_005 1246 CDS 100% 15.000 12.000 N DDX31 n/a
6 TRCN0000050368 CCACAATAAATACGGTCTTAA pLKO.1 880 CDS 100% 13.200 9.240 N DDX31 n/a
7 TRCN0000232435 CCACAATAAATACGGTCTTAA pLKO_005 880 CDS 100% 13.200 9.240 N DDX31 n/a
8 TRCN0000232437 GGACATCACAGTGATACTTAA pLKO_005 1352 CDS 100% 13.200 9.240 N DDX31 n/a
9 TRCN0000232439 ACTGCGCCTTTGCCTGAATAC pLKO_005 2799 3UTR 100% 10.800 7.560 N DDX31 n/a
10 TRCN0000050370 CCCTTCAAGCAATGGAGTCAA pLKO.1 1027 CDS 100% 4.950 3.465 N DDX31 n/a
11 TRCN0000184150 CGACACTCACAGAAGGTGTAA pLKO.1 1420 CDS 100% 4.950 3.465 N Ddx31 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_022779.8, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12496 pDONR223 100% 79.1% 79% None 1_531del;2395A>G n/a
2 ccsbBroad304_12496 pLX_304 0% 79.1% 79% V5 1_531del;2395A>G n/a
3 TRCN0000465632 GATACCCTCCTCTGCCCGTTCCAC pLX_317 18.1% 79.1% 79% V5 1_531del;2395A>G n/a
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