Construct: ORF TRCN0000465640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013202.1_s317c1
- Derived from:
- ccsbBroadEn_12846
- DNA Barcode:
- AGGGGGGCCACCAGTTGCCTGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRRC2B (84726)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84726 | PRRC2B | proline rich coiled-coil 2B | NM_013318.3 | 14.6% | 14.6% | 1_5709del |
| 2 | mouse | 227723 | Prrc2b | proline-rich coiled-coil 2B | XM_006497953.3 | 18.9% | 19.8% | (many diffs) |
| 3 | mouse | 227723 | Prrc2b | proline-rich coiled-coil 2B | XM_006497952.3 | 18.8% | 19.8% | (many diffs) |
| 4 | mouse | 227723 | Prrc2b | proline-rich coiled-coil 2B | NM_172661.3 | 15.4% | 15.7% | (many diffs) |
| 5 | mouse | 227723 | Prrc2b | proline-rich coiled-coil 2B | XM_006497950.3 | 13.2% | 13.9% | (many diffs) |
| 6 | mouse | 227723 | Prrc2b | proline-rich coiled-coil 2B | XM_006497949.3 | 13.2% | 13.8% | (many diffs) |
| 7 | mouse | 227723 | Prrc2b | proline-rich coiled-coil 2B | NM_001159634.1 | 13% | 13.7% | (many diffs) |
| 8 | mouse | 227723 | Prrc2b | proline-rich coiled-coil 2B | XM_006497948.3 | 13% | 13.7% | (many diffs) |
| 9 | mouse | 227723 | Prrc2b | proline-rich coiled-coil 2B | XM_006497951.3 | 10.6% | 10.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc acccatgcct gtggcctctg tagcaccttc tgcttctatg ccaggcagcc 121 acctcccgcc cctgtacctg gatggccatg tgtttgcaag tcagccccgg ctggttcctc 181 aaacgatacc tcagcagcag agttaccaac aggccgccgc tgcccagcag atcccgatct 241 cccttcacac atctctgcag gcacaagctc agcttggact gaggggtggg cttcctgtgt 301 cccagtccca ggagatcttc agctccttgc agcccttcag atctcaggtg tacatgcacc 361 ccagcctgtc accgcccagc accatgatcc tctctggggg cacagccttg aagcctccat 421 actcggcgtt cccaggcatg cagcccttgg agatggtgaa gccgcagtct ggctcaccct 481 accagcccat gagcgggaac caagccctgg tctacgaggg ccagctcagc caggctgctg 541 gcctgggtgc ctcccagatg ttggactccc agctcccaca gctgaccatg ccactgcctc 601 ggtacggctc cgggcagcag ccactgatcc tgccccagtc tattcagctg ccacctgggc 661 agagcctctc cgttggggcc ccccgaagga ttccTCCGCC CGGGTCCCAG CCGCCAGTCC 721 TGAACACCAG CAGAGAGCCC TCTCAGATGG AGATGAAAGG CTTCCACTTT GCCGACAGTA 781 AACAGAATGT CCCTTCAGGA GGCCCCGTGC CATCGCCACA GACCTACAGG CCTAGCTCTG 841 CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG CAGGGACACT 901 ACGTGCAACA GGCAAAACAA CGAGTGGATG AGAAACCCAG CCTGGGAGCC GTGAAGCTGC 961 AGGAGGCCCC CTCGGCTGCC TCCCAGATGA AGCGAACCGG AGCGATCAAG CCTCGGGCTG 1021 TCAAAGTGGA GGAGAGTAAG GCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GGGGGGCCAC 1201 CAGTTGCCTG AGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att