Construct: ORF TRCN0000465684
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001895.1_s317c1
- Derived from:
- ccsbBroadEn_03342
- DNA Barcode:
- CTGAATCTTTTTCCTGAGTGGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRMT6 (51605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465684
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51605 | TRMT6 | tRNA methyltransferase 6 | NM_015939.5 | 100% | 100% | |
| 2 | human | 51605 | TRMT6 | tRNA methyltransferase 6 | NM_001281467.1 | 65.7% | 65.7% | 0_1ins510 |
| 3 | human | 51605 | TRMT6 | tRNA methyltransferase 6 | XM_017027875.1 | 63.3% | 63.3% | 0_1ins546 |
| 4 | mouse | 66926 | Trmt6 | tRNA methyltransferase 6 | NM_175113.3 | 85.9% | 85.3% | (many diffs) |
| 5 | mouse | 66926 | Trmt6 | tRNA methyltransferase 6 | XM_006500051.2 | 55.9% | 55.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1557
- ORF length:
- 1491
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggctcaggg gagcagccgg gcccacaacc acagcatccc ggagaccacc 121 gcatccgcga cggcgacttc gtggtgctga aacgtgaaga tgtgtttaaa gcagtacaag 181 tccagcggag aaaaaaagta actttcgaaa aacagtggtt ctacctggat aacgtcattg 241 gccatagtta tggaactgca tttgaagtga ccagtggagg aagtctacag cccaagaaga 301 agagggaaga gcctactgca gagactaaag aagcgggcac tgataatcga aatatagttg 361 atgatgggaa atctcagaaa cttactcaag atgacataaa agctttgaag gacaagggca 421 ttaaaggaga ggaaatagtt cagcagttaa ttgaaaatag tacaacattc cgagacaaga 481 cagaatttgc ccaagataaa tatattaaaa agaagaaaaa aaaatatgaa gccatcatta 541 ctgttgtgaa gccatccacc cgtattcttt caattatgta ttatgcaaga gaacctggaa 601 aaattaacca catgagatac gatacactag cccagatgtt gacgttggga aatatccgtg 661 ctggcaacaa aatgattgtg atggaaacgt gtgcaggctt ggtgctgggt gcaatgatgg 721 aacgaatggg aggttttggc tccattattc agctataccc tggaggagga cctgttcggg 781 cagcaacagc atgttttgga tttcccaaat cttttctcag tggtctttat gaattccctc 841 tcaacaaagt ggacagtctt ctacatggaa cattttctgc caagatgtta tcttcagagc 901 caaaagacag tgctttggtt gaagaaagta atggcacact ggaggaaaaa caggcttctg 961 aacaagagaa tgaagacagc atggcagagg ccccagagag caaccaccca gaagaccagg 1021 aaacaatgga aacaatttct caagatccag aacataaggg gcctaaagag agaggaagca 1081 aaaaagatta tattcaggaa aaacagagga gacaagaaga gcagaggaaa agacatttag 1141 aggctgccgc TCTGCTGAGT GAAAGAAACG CAGATGGTTT AATTGTAGCT AGTCGTTTCC 1201 ACCCCACTCC CCTGCTGCTG TCTTTGCTGG ACTTTGTGGC CCCTTCAAGG CCGTTTGTGG 1261 TCTACTGTCA GTACAAAGAG CCTCTGTTGG AATGCTACAC AAAACTGCGG GAGAGGGGAG 1321 GGGTCATCAA CCTCAGGCTG TCTGAAACCT GGCTCAGAAA TTATCAGGTT TTGCCAGATC 1381 GAAGTCATCC TAAACTGCTG ATGAGTGGAG GTGGGGGTTA TCTTCTCTCC GGCTTCACCG 1441 TTGCCATGGA CAACCTTAAA GCAGACACCA GCCTCAAATC TAATGCAAGC ACTTTAGAAT 1501 CACACGAGAC TGAGGAGCCT GCAGCTAAAA AACGAAAATG CCCAGAGTCT GACTCTTACC 1561 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GACTGAATCT TTTTCCTGAG TGGAGGACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt