Construct: ORF TRCN0000465692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004576.1_s317c1
- Derived from:
- ccsbBroadEn_14107
- DNA Barcode:
- CGTATACTATCCAATCCCGAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF26A (26153)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26153 | KIF26A | kinesin family member 26A | XM_011536641.2 | 12.8% | 12.7% | 1_4110del;4117G>A;4127C>G |
| 2 | human | 26153 | KIF26A | kinesin family member 26A | NM_015656.1 | 10.7% | 10.6% | 1_5037del;5044G>A;5054C>G |
| 3 | mouse | 668303 | Kif26a | kinesin family member 26A | NM_001097621.1 | 9% | 9.3% | (many diffs) |
| 4 | mouse | 668303 | Kif26a | kinesin family member 26A | XM_006516162.2 | 8.8% | 9.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 675
- ORF length:
- 609
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caagagtggg ggtgcctccc caggcgcccg cacccgcagc ctcaagtccc 121 ccaagaagag ggccacaggt ctgcagcggc ggcgcctgat tcccgcccca ctgcccgaca 181 ccactgccct gggccgtaag cccagcctcc ccgggcagtg ggtggacctg cccccgcccc 241 tggctggctc cctgaaggag ccgttcgaga tcaaggtgta cgagatcgat gacgtggagc 301 gccTTCAGCG GCCCCGCCCC ACCCCGAGGG AGGCCCCCAC CCAGGGTCTG GCGTGCGTCA 361 GTACAAGGCT GCGGCTGGCG GAGCGCAGGC AGCAGCGGCT GCGGGAGGTG CAGGCCAAGC 421 ACAAGCACCT GTGTGAGGAG CTGGCCGAGA CCCAGGGCCG GCTGATGCTG GAGCCTGGCC 481 GCTGGCTGGA GCAGTTTGAG GTGGACCCGG AGCTGGAGCC CGAGTCGGCC GAGTACCTGG 541 CGGCCCTGGA GCGAGCCACG GCGGCCCTGG AGCAGTGCGT GAACCTGTGC AAGGCGCACG 601 TCATGATGGT CACCTGCTTC GACATCAGCG TTGCAGCCAG TGCTGCCATC CCGGGGCCGC 661 AGGAGGTGGA CGTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 721 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 781 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGTATACTAT CCAATCCCGA 841 ATTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt