Construct: ORF TRCN0000465697
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015662.1_s317c1
- Derived from:
- ccsbBroadEn_14436
- DNA Barcode:
- CCCGGGGAGAGCGAACGCCGAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ENKUR (219670)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465697
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219670 | ENKUR | enkurin, TRPC channel inter... | NM_145010.4 | 99.8% | 97.6% | 753delT |
| 2 | human | 219670 | ENKUR | enkurin, TRPC channel inter... | NM_001270383.1 | 74.5% | 69.1% | (many diffs) |
| 3 | human | 219670 | ENKUR | enkurin, TRPC channel inter... | NR_072992.2 | 39.5% | 1_222del;975delT;991_1939del | |
| 4 | mouse | 71233 | Enkur | enkurin, TRPC channel inter... | NM_027728.2 | 83.1% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 816
- ORF length:
- 750
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tccaacgtgc tcttctgagt gcatttataa cctcataccc agtgacttga 121 aggagcctcc ccagcctcct aggtacatat ccatttttaa ggcaactgta aaagatgaca 181 tgcaaaaagc taaaactgca atgaaaacta tgggaccagc aaaagttgaa gtaccttctc 241 caaaggattt cctaaagaaa cattcaaagg aaaaaactct accacccaaa aaaaactttg 301 atcggaacgt gcccaaaaag cctgctgtgc cattgaagac tgatcatcct gtcatgggaa 361 tacagagtgg aaaaaatttt ataaatacaa atgcagctga tatcatcatg ggagtggcta 421 aaaagcctaa accaatttat gttgataaaa gaactGGAGA CAAGCATGAT CTTGAGCCTT 481 CAGGACTAGT TCCAAAGTAC ATCAATAAAA AGGATTATGG TGTCACACCT GAATACATAT 541 GTAAGCGAAA CGAGGAAATA AAGAAAGCCC AAGAAGACTA TGATCGTTAT ATCCAGGAAA 601 ACCTTAAGAA AGCAGCTATG AAAAGGCTCT CCGATGAAGA AAGGGAGGCA GTTTTGCAGG 661 GGCTGAAAAA GAACTGGGAA GAGGTGCATA AAGAATTCCA GTCCCTCTCG GTCTTTATAG 721 ATTCTATACC AAAGAAGATC CGCAAGCAGA GGCTGGAAGA AGAAATGAAA CAACTAGAAC 781 ACGACATTGG CATAATTGAA AAGCACAAGA TTATTTAATT GCCAATAACG CATGCCCAAC 841 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 901 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 961 ATCTTGTGGA AAGGACGACC CGGGGAGAGC GAACGCCGAA TCACGCGTTA AGTCgacaat 1021 caacctctgg attacaaaat ttgtgaaaga tt