Construct: ORF TRCN0000465701
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018310.1_s317c1
- Derived from:
- ccsbBroadEn_06351
- DNA Barcode:
- TCAATTTGCCATGGTTGCTACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HARS1 (3035)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465701
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3035 | HARS1 | histidyl-tRNA synthetase 1 | NM_002109.6 | 99.9% | 99.8% | 96G>T |
| 2 | human | 3035 | HARS1 | histidyl-tRNA synthetase 1 | NM_001258041.2 | 96% | 95.8% | 96G>T;462_463ins60 |
| 3 | human | 3035 | HARS1 | histidyl-tRNA synthetase 1 | NM_001289094.1 | 94.1% | 93.9% | 1_1delAins88;9G>T |
| 4 | human | 3035 | HARS1 | histidyl-tRNA synthetase 1 | NM_001258040.2 | 92% | 91.9% | 96G>T;177_178ins120 |
| 5 | human | 3035 | HARS1 | histidyl-tRNA synthetase 1 | NM_001258042.2 | 88.1% | 88% | 96G>T;177_178ins120;342_343ins60 |
| 6 | human | 3035 | HARS1 | histidyl-tRNA synthetase 1 | NM_001289092.1 | 85.3% | 85.2% | 96G>T;300_301ins222 |
| 7 | human | 3035 | HARS1 | histidyl-tRNA synthetase 1 | NM_001289093.1 | 77.5% | 77.4% | 96G>T;180_181ins342 |
| 8 | mouse | 15115 | Hars | histidyl-tRNA synthetase | NM_008214.4 | 88.2% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1593
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagcgtgcg gcgctggagg agctggtgaa acttcaggga gagcgcgtgc 121 gaggcctcaa gcagcagaag gccagcgccg agctgatcga tgaggaggtg gcgaaactcc 181 tgaaactgaa ggcacagctg ggtcctgatg aaagcaaaca gaaatttgtg ctcaaaaccc 241 ccaagggcac aagagactat agtccccggc agatggcagt tcgcgagaag gtgtttgacg 301 taatcatccg ttgcttcaag cgccacggtg cagaagtcat tgatacacct gtatttgaac 361 taaaggaaac actgatggga aagtatgggg aagactccaa gcttatctat gacctgaagg 421 accagggcgg ggagctcctg tcccttcgct atgacctcac tgttcctttt gctcggtatt 481 tggcaatgaa taaactgacc aacattaaac gctaccacat agcaaaggta tatcggcggg 541 ataacccagc catgacccgt ggccgatacc gggaattcta ccagtgtgat tttgacattg 601 ctgggaactt tgatcccatg atccctgatg cagagtgcct gaagatcatg tgcgagatcc 661 tgagttcact tcagataggc gacttcctgg tcaaggtaaa cgatcgacgc attctagatg 721 ggatgtttgc tatctgtggt gtttctgaca gcaagttccg taccatctgc tcctcagtag 781 acaagctgga caaggtgtcc tgggaagagg tgaagaatga gatggtggga gagaagggcc 841 ttgcacctga ggtggctgac cgcattgggg actatgtcca gcaacatggt ggggtatccc 901 tggtggaaca gctgctccag gatcctaaac tatcccaaaa caagcaggcc ttggagggcc 961 tgggagacct gaagttgctc tttgagtacc tgaccctatt tggcattgat gacaaaatct 1021 cctttgacct gagccttgct cgagggctgg attactacac tggggtgatc tatgaggcag 1081 tgctgctaca gaccccagcc caggcagggg aagagcccct gggtgtgggc agtgtggctg 1141 ctggaggacg ctatgatggg ctagtgggca tgttcgaccc caaagggcgc aaggtgccat 1201 gtgtggggct cagcattgGG GTGGAGCGGA TTTTCTCCAT CGTGGAACAG AGACTAGAGG 1261 CTTTGGAGGA GAAGATACGG ACCACGGAGA CACAGGTGCT TGTGGCATCT GCACAGAAGA 1321 AGCTGCTAGA GGAAAGACTA AAGCTTGTCT CAGAACTGTG GGATGCTGGG ATCAAGGCTG 1381 AGCTGCTGTA CAAGAAGAAC CCAAAGCTAC TGAACCAGTT ACAGTACTGT GAGGAGGCAG 1441 GCATCCCACT GGTGGCTATC ATCGGCGAGC AGGAACTCAA GGATGGGGTC ATCAAGCTCC 1501 GTTCAGTGAC GAGCAGGGAA GAGGTGGATG TCCGAAGAGA AGACCTTGTG GAGGAAATCA 1561 AAAGGAGAAC AGGCCAGCCC CTCTGCATCT GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1741 AATTTGCCAT GGTTGCTACT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt