Transcript: Mouse NM_008214.4

Mus musculus histidyl-tRNA synthetase (Hars), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Hars (15115)
Length:
1999
CDS:
106..1635

Additional Resources:

NCBI RefSeq record:
NM_008214.4
NBCI Gene record:
Hars (15115)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008214.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000075904 CCAACATTAAACGCTACCATA pLKO.1 539 CDS 100% 4.950 6.930 N Hars n/a
2 TRCN0000306136 GATGTCATCATCCGCTGTTTC pLKO_005 337 CDS 100% 10.800 8.640 N Hars n/a
3 TRCN0000306137 GCTGCTCTTTGAGTATCTAAT pLKO_005 1014 CDS 100% 13.200 9.240 N Hars n/a
4 TRCN0000337578 TGAATAAACTGACCAACATTA pLKO_005 527 CDS 100% 13.200 9.240 N Hars n/a
5 TRCN0000075903 GTACTTAAACAGTCTGCATAT pLKO.1 1698 3UTR 100% 10.800 7.560 N Hars n/a
6 TRCN0000325938 GTACTTAAACAGTCTGCATAT pLKO_005 1698 3UTR 100% 10.800 7.560 N Hars n/a
7 TRCN0000075906 CCCGAAGTTACTAAACCAGTT pLKO.1 1440 CDS 100% 4.050 2.835 N Hars n/a
8 TRCN0000325940 CCCGAAGTTACTAAACCAGTT pLKO_005 1440 CDS 100% 4.050 2.835 N Hars n/a
9 TRCN0000075907 CCTGTGTTTGAACTAAAGGAA pLKO.1 388 CDS 100% 3.000 2.100 N Hars n/a
10 TRCN0000325939 CCTGTGTTTGAACTAAAGGAA pLKO_005 388 CDS 100% 3.000 2.100 N Hars n/a
11 TRCN0000075905 CGCATCCTAGATGGGATGTTT pLKO.1 748 CDS 100% 0.563 0.394 N Hars n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008214.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06351 pDONR223 100% 88.2% 94.6% None (many diffs) n/a
2 ccsbBroad304_06351 pLX_304 0% 88.2% 94.6% V5 (many diffs) n/a
3 TRCN0000465701 TCAATTTGCCATGGTTGCTACTTT pLX_317 24.8% 88.2% 94.6% V5 (many diffs) n/a
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