Construct: ORF TRCN0000465706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004611.1_s317c1
- Derived from:
- ccsbBroadEn_00209
- DNA Barcode:
- TCCGATATATCCAATCTGGCCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALML3 (810)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 810 | CALML3 | calmodulin like 3 | NM_005185.4 | 100% | 100% | |
| 2 | mouse | 70405 | Calml3 | calmodulin-like 3 | NM_027416.3 | 84.3% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgaccagctg actgaggagc aggtcacaga attcaaggag gccttctccc 121 tgtttgacaa ggatggggac ggctgcatca ccacccgcga gctgggcacg gtcatgcggt 181 cccTGGGCCA GAACCCCACG GAGGCCGAGC TGCGGGACAT GATGAGTGAG ATCGACCGGG 241 ACGGCAACGG CACCGTGGAC TTCCCCGAGT TCCTGGGCAT GATGGCCAGG AAGATGAAGG 301 ACACGGACAA CGAGGAGGAG ATCCGCGAGG CCTTCCGCGT GTTCGACAAG GACGGCAACG 361 GCTTCGTCAG CGCCGCCGAG CTGCGACACG TCATGACCCG GCTGGGGGAG AAGCTGAGTG 421 ACGAGGAGGT GGACGAGATG ATCCGGGCCG CGGACACGGA CGGAGACGGA CAGGTGAACT 481 ACGAGGAGTT TGTCCGTGTG CTGGTGTCCA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 661 CGATATATCC AATCTGGCCC CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt