Construct: ORF TRCN0000465707
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016621.1_s317c1
- Derived from:
- ccsbBroadEn_15816
- DNA Barcode:
- ATTTAAAGGTTACAATCGGCATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMED5 (50999)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465707
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50999 | TMED5 | transmembrane p24 trafficki... | NM_016040.5 | 100% | 100% | |
| 2 | human | 50999 | TMED5 | transmembrane p24 trafficki... | NM_001167830.2 | 72% | 71.2% | 470_518del;579_580ins157 |
| 3 | human | 50999 | TMED5 | transmembrane p24 trafficki... | NR_030761.2 | 11.7% | 1_171del;459_529del;930_5860del | |
| 4 | mouse | 73130 | Tmed5 | transmembrane p24 trafficki... | NM_028876.2 | 89% | 89.9% | (many diffs) |
| 5 | mouse | 73130 | Tmed5 | transmembrane p24 trafficki... | XM_006535238.2 | 74.9% | 75.5% | (many diffs) |
| 6 | mouse | 73130 | Tmed5 | transmembrane p24 trafficki... | XM_006535240.2 | 40.1% | 36.7% | (many diffs) |
| 7 | mouse | 73130 | Tmed5 | transmembrane p24 trafficki... | NM_001347383.1 | 37.7% | 36.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgacaagatc tggctgccct tccccgtgct ccttctggcc gctctgcctc 121 cggtgctgct gcctggggcg gccggcttca caccttccct cgatagcgac ttcaccttta 181 cccttcccgc cggccagaag gagtgcttct accagcccat gcccctgaag gcctcgctgg 241 agatcgagta ccaagtttta gatggagcag gattagatat tgatttccat cttgcctctc 301 cagaaggcaa aaccttagtt tttgaacaaa gaaaatcaga tggagttcac actgtagaga 361 ctgaagttgg tgattacatg ttctgctttg acaatacatt cagcaccatt tctgagaagg 421 tgattttctt tgaattaatc ctggataata tgggagaaca ggcacaagaa caagaagatt 481 ggaagaaata tattactggc acagatatat tGGATATGAA ACTGGAAGAC ATCCTGGAAT 541 CCATCAACAG CATCAAGTCC AGACTAAGCA AAAGTGGGCA CATACAAACT CTGCTTAGAG 601 CATTTGAAGC TCGTGATCGA AACATACAAG AAAGCAACTT TGATAGAGTC AATTTCTGGT 661 CTATGGTTAA TTTAGTGGTC ATGGTGGTGG TGTCAGCCAT TCAAGTTTAT ATGCTGAAGA 721 GTCTGTTTGA AGATAAGAGG AAAAGTAGAA CTTACCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 901 TTAAAGGTTA CAATCGGCAT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt