Transcript: Human NM_001167830.2

Homo sapiens transmembrane p24 trafficking protein 5 (TMED5), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
TMED5 (50999)
Length:
5838
CDS:
172..753

Additional Resources:

NCBI RefSeq record:
NM_001167830.2
NBCI Gene record:
TMED5 (50999)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001167830.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000065121 GAGCAGGATTAGATATTGATT pLKO.1 371 CDS 100% 5.625 7.875 N TMED5 n/a
2 TRCN0000307125 GAGCAGGATTAGATATTGATT pLKO_005 371 CDS 100% 5.625 7.875 N TMED5 n/a
3 TRCN0000065119 GCAACTTTGATAGAGTCAATT pLKO.1 789 3UTR 100% 13.200 9.240 N TMED5 n/a
4 TRCN0000289698 GCAACTTTGATAGAGTCAATT pLKO_005 789 3UTR 100% 13.200 9.240 N TMED5 n/a
5 TRCN0000065118 GCCTAGAGTTTGCTCTGATAT pLKO.1 3377 3UTR 100% 13.200 9.240 N TMED5 n/a
6 TRCN0000289697 GCCTAGAGTTTGCTCTGATAT pLKO_005 3377 3UTR 100% 13.200 9.240 N TMED5 n/a
7 TRCN0000065122 GAACAAGAAGATTGGAAGAAA pLKO.1 574 CDS 100% 5.625 3.938 N TMED5 n/a
8 TRCN0000289694 GAACAAGAAGATTGGAAGAAA pLKO_005 574 CDS 100% 5.625 3.938 N TMED5 n/a
9 TRCN0000065120 GACAATACATTCAGCACCATT pLKO.1 496 CDS 100% 4.950 3.465 N TMED5 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001167830.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15816 pDONR223 0% 72% 71.2% None 470_518del;579_580ins157 n/a
2 ccsbBroad304_15816 pLX_304 0% 72% 71.2% V5 470_518del;579_580ins157 n/a
3 TRCN0000465707 ATTTAAAGGTTACAATCGGCATGT pLX_317 35.6% 72% 71.2% V5 470_518del;579_580ins157 n/a
4 ccsbBroadEn_08192 pDONR223 100% 71.8% 72.4% None 470_518del;573C>T;579_580ins157 n/a
5 ccsbBroad304_08192 pLX_304 0% 71.8% 72.4% V5 470_518del;573C>T;579_580ins157 n/a
6 TRCN0000465252 ATTATGAATCCCTAAAAGGTGACA pLX_317 35.6% 71.8% 72.4% V5 470_518del;573C>T;579_580ins157 n/a
Download CSV