Construct: ORF TRCN0000465708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017901.1_s317c1
- Derived from:
- ccsbBroadEn_10274
- DNA Barcode:
- ACATTACCATATTGCCCGAATTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBRG1 (84897)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465708
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84897 | TBRG1 | transforming growth factor ... | NM_032811.3 | 33.8% | 31.4% | 1_63del;453_589del;618_1233del |
| 2 | human | 84897 | TBRG1 | transforming growth factor ... | XM_017018442.2 | 15.8% | 3.6% | 0_1ins253;165_780del |
| 3 | human | 84897 | TBRG1 | transforming growth factor ... | NR_016021.2 | 8.3% | 1_283del;701_5007del | |
| 4 | human | 84897 | TBRG1 | transforming growth factor ... | XR_428983.4 | 8% | 1_287del;677_872del;901_5205del | |
| 5 | human | 84897 | TBRG1 | transforming growth factor ... | XR_001748012.2 | 7.7% | (many diffs) | |
| 6 | human | 84897 | TBRG1 | transforming growth factor ... | XR_001748011.2 | 7.6% | (many diffs) | |
| 7 | mouse | 21376 | Tbrg1 | transforming growth factor ... | NM_025289.3 | 30% | 27.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 486
- ORF length:
- 417
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaaagctc ccgaagaaga gccagaatga gaagtaccgg ctgaagtacc 121 TGCGGCTGCG CAAAGCGGCC AAGGCCACGG TGTTTGAAAA TGCTGCTATT TGTGATGAAA 181 TTGCTCGTCT TGAGGAAAAA TTTCTTAAAG CAAAAGAAGA AAGAAGGTAC TTGCTAAAGA 241 AGCTCCTCCA GCTTCAGGCT CTAACTGAAG GGGAAGTACA GGCTGCAGCT CCTTCCCACA 301 GTTCCAGTTT GCCCCTGACT TATGGTGTGG CCAGCTCTGT GGGAACTATA CAGGGAGCTG 361 GGCCTATTTC AGGGCCCAGC ACTGGGGCTG AGGAACCATT TGGGAAGAAA ACTAAGAAGG 421 AGAAAAAAGA AAAAGGCAAA GAGAACAACA AACTGGAAGA TCATCACCGA CCGACCTGGC 481 TTTCATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACATTACCA TATTGCCCGA ATTGGACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt