Construct: ORF TRCN0000465713
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014469.1_s317c1
- Derived from:
- ccsbBroadEn_14119
- DNA Barcode:
- TATAGTTTGTCCTTGCTAAACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INVS (27130)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465713
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27130 | INVS | inversin | NM_014425.5 | 9.3% | 8.7% | (many diffs) |
| 2 | human | 27130 | INVS | inversin | NR_134606.1 | 6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 393
- ORF length:
- 303
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAACAAGTC AGAGAACCTG CTGTTTGCTG 121 GTTCATCATT AGCATCACAA GTCCATGCTG CTGCCGTTAA TGGAGATAAG GGTGCTCTAC 181 AGAGGCTCAT CGTAGGAAAC TCTGCTCTTA AAGACAAAGA AGATCAGTTT GGGAGAACAC 241 CACTTATGTA TTGCGTGTTG GCTGACAGAT TGGATTGTGC AGATGCTCTT CTGAAGGCAG 301 GAGCAGATGT GAATAAAACT GACCATAGCC AGAGAACAGC CCTCCATCTT GCAGCCCAGA 361 AGGCATTGAG AACAATCCGC ACAGGCAGGA TCTACCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 541 TAGTTTGTCC TTGCTAAACA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt