Construct: ORF TRCN0000465715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015359.1_s317c1
- Derived from:
- ccsbBroadEn_12734
- DNA Barcode:
- CAGCATGCTCTGCGTCTCATCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABHD17A (81926)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465715
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81926 | ABHD17A | abhydrolase domain containi... | NM_001130111.2 | 75.9% | 75.4% | 293G>A;708_930delinsG |
| 2 | human | 81926 | ABHD17A | abhydrolase domain containi... | NM_031213.4 | 65.1% | 64.8% | 293G>A;328_480del;861_1083delinsG |
| 3 | human | 81926 | ABHD17A | abhydrolase domain containi... | XM_011528338.2 | 65.1% | 64.8% | 293G>A;328_480del;861_1083delinsG |
| 4 | human | 81926 | ABHD17A | abhydrolase domain containi... | XR_001753771.1 | 39.1% | (many diffs) | |
| 5 | mouse | 216169 | Abhd17a | abhydrolase domain containi... | NM_145421.2 | 65.9% | 68.3% | (many diffs) |
| 6 | mouse | 216169 | Abhd17a | abhydrolase domain containi... | XM_017313894.1 | 65.9% | 68.3% | (many diffs) |
| 7 | mouse | 216169 | Abhd17a | abhydrolase domain containi... | XM_006513504.2 | 47.9% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgggctgtcg ctgagtgagc tctgctgcct cttctgctgc ccgccctgcc 121 ccggccgcat cgctgccaag ctcgccttcc tgccgccgga ggccacctac tccctggtgc 181 ctgagcccga gccggggcct ggtggggccg gggccgcccc cttggggacc ctgagagcct 241 cctcgggcgc acccgggcgc tggaagctgc acctgacgga gcgtgccgac ttccagtaca 301 gccagcgcga gctggacacc atcgaggtct tccccaccaa gagcgcccgc ggcaaccacg 361 tctcctgcat gtatgttcgc tgcgtgcctg gtgccaggta cacggTCCTC TTCTCGCACG 421 GCAATGCCGT GGACCTGGGC CAGATGAGCA GCTTCTACAT TGGCCTGGGC TCCCGCCTCC 481 ACTGCAACAT CTTCTCCTAC GACTACTCCG GCTACGGTGC CAGCTCGGGC AGGCCTTCCG 541 AGAGGAACCT CTATGCCGAC ATCGACGCCG CCTGGCAGGC CCTGCGCACC AGGTACGGCA 601 TCAGCCCGGA CAGCATCATC CTGTACGGGC AGAGCATCGG CACGGTGCCC ACCGTGGACC 661 TGGCCTCGCG CTACGAGTGT GCCGCGGTGG TGCTGCACTC GCCGCTCACC TCGGGCATGC 721 GCGTCGCCTT CCCCGACACC AAGAAGACCT ACTGCTTCGA CGCCTTCCCT AAGTGCCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAC AGCATGCTCT GCGTCTCATC CAGACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att