Construct: ORF TRCN0000465733
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007323.1_s317c1
- Derived from:
- ccsbBroadEn_12037
- DNA Barcode:
- GTCATCTTCTTCCTGACTGTTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YIPF1 (54432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465733
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54432 | YIPF1 | Yip1 domain family member 1 | NM_018982.5 | 40.1% | 40.1% | 1_549del |
| 2 | human | 54432 | YIPF1 | Yip1 domain family member 1 | NR_135075.2 | 24.2% | 1_603del;973_1521del | |
| 3 | human | 54432 | YIPF1 | Yip1 domain family member 1 | NR_036640.2 | 22.3% | 1_683del;1053_1652del | |
| 4 | human | 54432 | YIPF1 | Yip1 domain family member 1 | NR_036639.2 | 19.7% | 1_903del;1273_1872del | |
| 5 | mouse | 230584 | Yipf1 | Yip1 domain family, member 1 | NM_001205156.1 | 37.6% | 38.3% | (many diffs) |
| 6 | mouse | 230584 | Yipf1 | Yip1 domain family, member 1 | NM_145550.3 | 34.3% | 34.9% | (many diffs) |
| 7 | mouse | 230584 | Yipf1 | Yip1 domain family, member 1 | XM_006502968.3 | 27.3% | 27.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 435
- ORF length:
- 369
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gagaaacagc aaagttatga acatcgtctc ctattcattt ctggagattg 121 tgtgtgtcta tggatattcc ctcttcattt atatccccac cgcaatactg tggattatcc 181 cccagaaagc tgttcgttgg attctagtca tgattgccct gggcatctca ggatctctct 241 tggcaatgac attttggcca gctgttcgtg aggataaccg acgcGTTGCA TTGGCCACAA 301 TTGTGACAAT TGTGTTGCTC CATATGCTGC TTTCTGTGGG CTGCTTGGCA TACTTTTTTG 361 ATGCACCAGA GATGGACCAT CTCCCAACAA CTACAGCTAC TCCAAACCAA ACAGTTGCTG 421 CAGCCAAGTC CAGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 481 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 541 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTCATCTTCT TCCTGACTGT 601 TAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt