Transcript: Human NR_135075.2

Homo sapiens Yip1 domain family member 1 (YIPF1), transcript variant 4, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
YIPF1 (54432)
Length:
1521
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_135075.2
NBCI Gene record:
YIPF1 (54432)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_135075.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000144885 GTTATGAACATCGTCTCCTAT pLKO.1 622 3UTR 100% 4.950 6.930 N YIPF1 n/a
2 TRCN0000139337 CGTACCATTATGTGCCCGAAT pLKO.1 506 3UTR 100% 4.050 5.670 N YIPF1 n/a
3 TRCN0000145307 CCTGAAATAGAACCATGGAAA pLKO.1 1309 3UTR 100% 4.950 3.960 N YIPF1 n/a
4 TRCN0000140268 GCCACCACAGTCAAATGACAT pLKO.1 1266 3UTR 100% 4.950 3.960 N YIPF1 n/a
5 TRCN0000143720 GAGGTTATATATCCGCAGCAA pLKO.1 384 3UTR 100% 2.640 2.112 N YIPF1 n/a
6 TRCN0000122045 CAGGTCTTTGACAGAATTAAA pLKO.1 328 3UTR 100% 15.000 10.500 N YIPF1 n/a
7 TRCN0000144731 GATGATGAGTTACTGGGAAAT pLKO.1 208 3UTR 100% 10.800 7.560 N YIPF1 n/a
8 TRCN0000143501 GCCATAGCAATTAGTGGGAAT pLKO.1 451 3UTR 100% 4.050 2.835 N YIPF1 n/a
9 TRCN0000143500 GTGACAATTGTGTTGCTCCAT pLKO.1 841 3UTR 100% 2.640 1.848 N YIPF1 n/a
10 TRCN0000139151 CCAAACCAAACAGTTGCTGCA pLKO.1 940 3UTR 100% 2.160 1.512 N YIPF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_135075.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03414 pDONR223 100% 59.3% None (many diffs) n/a
2 ccsbBroad304_03414 pLX_304 0% 59.3% V5 (many diffs) n/a
3 TRCN0000467922 CTCCGACATGTTCATGGGGTGAAG pLX_317 45.8% 59.3% V5 (many diffs) n/a
4 ccsbBroadEn_12037 pDONR223 100% 24.2% None 1_603del;973_1521del n/a
5 ccsbBroad304_12037 pLX_304 0% 24.2% V5 1_603del;973_1521del n/a
6 TRCN0000465733 GTCATCTTCTTCCTGACTGTTAAT pLX_317 41.3% 24.2% V5 1_603del;973_1521del n/a
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