Construct: ORF TRCN0000465750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014306.1_s317c1
- Derived from:
- ccsbBroadEn_14038
- DNA Barcode:
- CAATCGATTGGTATATCGAAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USH1C (10083)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10083 | USH1C | USH1 protein network compon... | NM_001297764.2 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 10083 | USH1C | USH1 protein network compon... | NM_005709.4 | 96.3% | 96.1% | (many diffs) |
| 3 | human | 10083 | USH1C | USH1 protein network compon... | XM_011519832.3 | 88.1% | 88% | (many diffs) |
| 4 | human | 10083 | USH1C | USH1 protein network compon... | XM_017017074.1 | 79.7% | 72.2% | (many diffs) |
| 5 | human | 10083 | USH1C | USH1 protein network compon... | XM_011519834.2 | 79% | 75% | (many diffs) |
| 6 | human | 10083 | USH1C | USH1 protein network compon... | XR_930842.1 | 72.3% | (many diffs) | |
| 7 | human | 10083 | USH1C | USH1 protein network compon... | XR_930841.1 | 70.2% | (many diffs) | |
| 8 | human | 10083 | USH1C | USH1 protein network compon... | XR_001747717.2 | 69.7% | (many diffs) | |
| 9 | human | 10083 | USH1C | USH1 protein network compon... | NR_123738.2 | 68.1% | (many diffs) | |
| 10 | human | 10083 | USH1C | USH1 protein network compon... | XM_017017073.1 | 67.2% | 58.1% | (many diffs) |
| 11 | human | 10083 | USH1C | USH1 protein network compon... | XM_017017072.1 | 65.5% | 56.6% | (many diffs) |
| 12 | human | 10083 | USH1C | USH1 protein network compon... | NM_153676.4 | 58.8% | 57.5% | (many diffs) |
| 13 | human | 10083 | USH1C | USH1 protein network compon... | XM_017017075.1 | 55.3% | 54.1% | (many diffs) |
| 14 | mouse | 72088 | Ush1c | USH1 protein network compon... | NM_023649.2 | 85% | 91.1% | (many diffs) |
| 15 | mouse | 72088 | Ush1c | USH1 protein network compon... | XM_006541212.3 | 79.9% | 85.5% | (many diffs) |
| 16 | mouse | 72088 | Ush1c | USH1 protein network compon... | NM_001291182.1 | 55.1% | 57.8% | (many diffs) |
| 17 | mouse | 72088 | Ush1c | USH1 protein network compon... | NM_001163733.1 | 53% | 55.7% | (many diffs) |
| 18 | mouse | 72088 | Ush1c | USH1 protein network compon... | NM_153677.2 | 51.9% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1665
- ORF length:
- 1599
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccgaaaagtg gcccgagaat tccggcataa ggtggatttt ctgattgaaa 121 atgatgcaga gaaggactat ctctatgatg tgctgcgaat gtaccaccag accatggacg 181 tggccgtgct cgtgggagac ctgaagctgg tcatcaatga acccagccgt ctgcctctgt 241 ttgatgccat tcggccgctg atcccactga agcaccaggt ggaatatgat cagctgaccc 301 cccggcgctc caggaagctg aaggaggtgc gtctggaccg tctgcacccc gaaggcctcg 361 gcctgagtgt gcgtggtggc ctggagtttg gctgtgggct cttcatctcc cacctcatca 421 aaggcggtca ggcagacagc gtcgggctcc aggtagggga cgagatcgtc cggatcaatg 481 gatattccat ctcctcctgt acccatgagg aggtcatcaa cctcattcga accaagaaaa 541 ctgtgtccat caaagtgaga cacatcggcc tgatccccgt gaaaagctct cctgatgagc 601 ccctcacttg gcagtatgtg gatcagtttg tgtcggaatc tgggggcgtg cgaggcagcc 661 tgggctcccc tggaaatcgg gaaaacaagg agaagaaggt cttcatcagc ctggtaggct 721 cccgaggcct tggctgcagc atttccagcg gccccatcca gaagcctggc atctttatca 781 gccatgtgaa acctggctcc ctgtctgctg aggtgggatt ggagataggg gaccagattg 841 tcgaagtcaa tggcgtcgac ttctctaacc tggatcacaa ggagggccgg gagctgttca 901 tgacagaccg ggagcggctg gcagaggcgc ggcagcgtga gctgcagcgg caggagcttc 961 tcatgcagaa gcggctggcg atggagtcca acaagatcct ccaggagcag caggagatgg 1021 agcggcaaag gagaaaagaa attgcccaga aggcagcaga ggaaaatgag agataccgga 1081 aggagatgga acagattgta gaggaggaag agaagtttaa gaagcaatgg gaagaagact 1141 ggggctcaaa ggaacagcta ctcttgccta aaaccatcac tgctgaggta cacccggtac 1201 cccttcgcaa gccaaagtat gatcagggag tggaacctga gctcgagccc gcagatgacc 1261 tggatggagg cacggaggag cagggagagc aggattTCCG GAAATATGAG GAAGGCTTTG 1321 ACCCCTACTC TATGTTCACC CCAGAGCAGA TCATGGGGAA GGATGTCCGG CTCCTACGCA 1381 TCAAGAAGGA GGGATCCTTA GACCTGGCCC TGGAAGGCGG TGTGGACTCC CCCATTGGGA 1441 AGGTGGTTGT TTCTGCTGTG TATGAGCGGG GAGCTGCTGA GCGGCATGGT GGCATTGTGA 1501 AAGGGGACGA GATCATGGCA ATCAACGGCA AGATTGTGAC AGACTACACC CTGGCTGAGG 1561 CTGACGCTGC CCTGCAGAAG GCCTGGAATC AGGGCGGGGA CTGGATCGAC CTTGTGGTTG 1621 CCGTCTGCCC CCCAAAGGAG TATGACGATG AGCTGACCCT CTTCTGCCCA ACTTTCTTGT 1681 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1741 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1801 GAAAGGACGA CAATCGATTG GTATATCGAA ATTCACGCGT TAAGTCgaca atcaacctct 1861 ggattacaaa atttgtgaaa gatt