Construct: ORF TRCN0000465758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015335.1_s317c1
- Derived from:
- ccsbBroadEn_06659
- DNA Barcode:
- CACGTAATGAGGTCTGCATAATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NTF3 (4908)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465758
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4908 | NTF3 | neurotrophin 3 | NM_002527.4 | 99.7% | 100% | 252G>A;555C>T |
| 2 | human | 4908 | NTF3 | neurotrophin 3 | XM_011520963.2 | 99.7% | 100% | 252G>A;555C>T |
| 3 | human | 4908 | NTF3 | neurotrophin 3 | NM_001102654.2 | 94.9% | 95.1% | 1_39del;291G>A;594C>T |
| 4 | mouse | 18205 | Ntf3 | neurotrophin 3 | NM_008742.3 | 89.5% | 95.7% | (many diffs) |
| 5 | mouse | 18205 | Ntf3 | neurotrophin 3 | XM_006505716.2 | 89.5% | 95.7% | (many diffs) |
| 6 | mouse | 18205 | Ntf3 | neurotrophin 3 | NM_001164034.1 | 85.2% | 91.1% | (many diffs) |
| 7 | mouse | 18205 | Ntf3 | neurotrophin 3 | NM_001164035.1 | 85.2% | 91.1% | (many diffs) |
| 8 | mouse | 18205 | Ntf3 | neurotrophin 3 | XM_006505715.2 | 83% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 840
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccatcttg ttttatgtga tatttctcgc ttatctccgt ggcatccaag 121 gtaacaacat ggatcaaagg agtttgccag aagactcgct caattccctc attattaagc 181 tgatccaggc agatattttg aaaaacaagc tctccaagca gatggtggac gttaaggaaa 241 attaccagag caccctgccc aaagctgagg ctccccgaga gccggagcgg ggagggcccg 301 ccaagtcagc attccagcca gtgattgcaa tggacaccga actgctgcga caacagagac 361 gctacaactc accgcgggtc ctgctgagcg acagcacccc cttggagccc ccgcccttgt 421 atctcatgga ggattacgtg ggcagccccg tggtggcgaa cagaacatca cggcggaaac 481 ggtacgcgga gcataagagt CACCGAGGGG AGTACTCGGT ATGTGACAGT GAGAGTCTGT 541 GGGTGACCGA CAAGTCATCG GCCATCGACA TTCGGGGACA CCAGGTCACG GTGCTGGGGG 601 AGATCAAAAC GGGCAACTCT CCTGTCAAAC AATATTTTTA TGAAACGCGA TGTAAGGAAG 661 CCAGGCCGGT CAAAAACGGT TGCAGGGGTA TTGATGATAA ACACTGGAAC TCTCAGTGCA 721 AAACATCCCA AACCTACGTC CGAGCACTGA CTTCAGAGAA CAATAAACTC GTGGGCTGGC 781 GGTGGATACG GATAGACACG TCCTGTGTGT GTGCCTTGTC GAGAAAAATC GGAAGAACAT 841 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGACACGT AATGAGGTCT GCATAATCTA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt