Construct: ORF TRCN0000465760
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006650.2_s317c1
- Derived from:
- ccsbBroadEn_14011
- DNA Barcode:
- CCATCGCCATTCATCGTGCCCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAPSS1 (9061)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465760
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | XM_011532400.2 | 99.9% | 99.8% | 14C>A |
| 2 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | XM_011532401.1 | 99.9% | 99.8% | 14C>A |
| 3 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | NM_005443.5 | 96.5% | 96.4% | 1_63del;77C>A |
| 4 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289477.1 | 87.7% | 98.5% | (many diffs) |
| 5 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289478.1 | 87.7% | 98.5% | (many diffs) |
| 6 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_011863.2 | 84.8% | 95.1% | (many diffs) |
| 7 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289479.1 | 80.5% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1875
- ORF length:
- 1809
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gagagcaaac aatgtcacct accaagccca tcatgtcagc aggaacaaga 121 gaggtcaggt ggtggggacc agaggtggct ttcgtggttg cacagtttgg ctaacaggct 181 tgtctggagc gggaaagact actgtgagca tggccttgga ggagtacctg gtttgtcatg 241 gtattccatg ctacactctg gatggtgaca atattcgtca aggtctcaat aaaaatcttg 301 gctttagtcc tgaagacaga gaagagaatg ttcgacgcat cgcagaagtt gctaaactgt 361 ttgcagatgc tggcttagtg tgcatcacaa gtttcatatc accttacact caggatcgca 421 acaatgcaag gcaaattcat gaaggtgcaa gtttaccgtt ttttgaagta tttgttgatg 481 ctcctctgca tgtttgtgaa cagagggatg tcaaaggact ctacaaaaaa gcccgggcag 541 gagaaattaa aggtttcact gggatcgatt ctgaatatga aaagccagag gcccctgagt 601 tggtgctgaa aacagactcc tgtgatgtaa atgactgtgt ccagcaagtt gtggaacttc 661 tacaggaacg ggatattgta cctgtggatg catcttatga agtaaaagaa ctatatgtgc 721 cagaaaataa acttcatttg gcaaaaacag atgcggaaac attaccagca ctgaaaatta 781 ataaagtgga tatgcagtgg gtgcaggttt tggcagaagg ttgggcaacc ccattgaatg 841 gctttatgag agagagggag tacttgcagt gccttcattt tgattgtctt ctggatggag 901 gtgtcattaa cttgtcagta cctatagttc tgactgcgac tcatgaagat aaagagaggc 961 tggacggctg tacagcattt gctctgatgt atgagggccg ccgtgtggcc attcttcgca 1021 atccagagtt ttttgagcac aggaaagagg agcgctgtgc cagacagtgg ggaacgacat 1081 gcaagaacca cccctatatt aagatggtga tggaacaagg agattggctg attggaggag 1141 atcttcaagt cttggatcga gtttattgga atgatggtct tgatcagtat cgtcttactc 1201 ctactgagct aaagcagaaa tttaaagata tgaatgctga tgctgtcttt gcatttcaac 1261 tacgcaaccc agtgcacaat ggacatgccc tgttaatgca ggatacccat aagcaacttc 1321 tagagagggg ctaccggcgc cctgtcctcc tcctccaccc tctgggtggc tggacaaagg 1381 atgacgatgt tcctttgatg tggcgtatga agcagcatgc tgcagtgttg gaggaaggag 1441 ttctgaatcc tgagacgaca gtggtggcca tcttcccaTC TCCCATGATG TATGCTGGAC 1501 CAACTGAGGT CCAGTGGCAT TGCAGAGCAC GGATGGTTGC AGGAGCCAAC TTTTACATTG 1561 TTGGACGAGA CCCTGCTGGC ATGCCTCATC CAGAAACAGG GAAGGATCTT TATGAGCCAA 1621 GTCATGGTGC CAAAGTGCTG ACGATGGCCC CTGGTTTAAT CACTTTGGAA ATAGTTCCCT 1681 TTCGAGTTGC AGCTTACAAC AAGAAAAAGA AGCGTATGGA CTACTATGAC TCTGAACACC 1741 ATGAAGACTT TGAATTTATT TCAGGAACAC GAATGCGCAA ACTTGCTCGA GAAGGCCAGA 1801 AACCACCTGA AGGTTTCATG GCTCCCAAGG CTTGGACCGT GCTGACAGAA TACTACAAAT 1861 CCTTGGAGAA AGCTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1921 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1981 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCATCGCCAT TCATCGTGCC 2041 CCGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt