Construct: ORF TRCN0000465761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014099.1_s317c1
- Derived from:
- ccsbBroadEn_07389
- DNA Barcode:
- GGTATCCGCGATAGTTCGACGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF3C5 (9328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465761
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9328 | GTF3C5 | general transcription facto... | NM_012087.4 | 99.8% | 100% | 6G>T;1554C>T |
| 2 | human | 9328 | GTF3C5 | general transcription facto... | NM_001122823.2 | 98.5% | 98.6% | 6G>T;1167_1187del;1575C>T |
| 3 | human | 9328 | GTF3C5 | general transcription facto... | XM_017015316.1 | 90.8% | 90.1% | (many diffs) |
| 4 | human | 9328 | GTF3C5 | general transcription facto... | XM_017015315.1 | 89.6% | 88.9% | (many diffs) |
| 5 | human | 9328 | GTF3C5 | general transcription facto... | NM_001286709.1 | 65.4% | 62.5% | (many diffs) |
| 6 | human | 9328 | GTF3C5 | general transcription facto... | XR_002956827.1 | 46.3% | (many diffs) | |
| 7 | human | 9328 | GTF3C5 | general transcription facto... | XR_002956826.1 | 45% | (many diffs) | |
| 8 | mouse | 70239 | Gtf3c5 | general transcription facto... | NM_001290484.1 | 83.5% | 85.1% | (many diffs) |
| 9 | mouse | 70239 | Gtf3c5 | general transcription facto... | NM_148928.3 | 82.7% | 84.4% | (many diffs) |
| 10 | mouse | 70239 | Gtf3c5 | general transcription facto... | XM_011239177.2 | 63.7% | 65.5% | (many diffs) |
| 11 | mouse | 70239 | Gtf3c5 | general transcription facto... | XM_017319253.1 | 62.8% | 64.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1623
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 taggcatggc tgcggaggcg gccgatttgg ggctgggggc cgccgtcccc gtggagctga 121 ggcgggagcg acgcatggtg tgcgtggagt acccgggagt ggtgcgtgat gtggctaaga 181 tgctgccgac tctgggcggc gaggaaggcg tctcccggat ctacgcagac cccaccaaga 241 ggctggagct gtacttccgg cccaaggacc catactgcca cccagtgtgc gccaaccgct 301 tcagtaccag cagcctgctg ctccgcatca ggaagagaac gaggcggcag aaaggggtgc 361 tgggcactga ggcccactcc gaggtcacat ttgacatgga gatccttggc atcatctcca 421 ccatttacaa atttcagggg atgtctgact tccagtactt ggctgtgcat acggaagcag 481 gcggcaagca tacgtcaatg tatgacaagg tgctcatgct ccggcccgag aaggaggcct 541 ttttccacca ggagctgccg ctctacatcc ccccacccat cttctcccgg ctggacgccc 601 cggtggacta cttctaccga ccagagaccc agcaccggga aggctacaac aatcccccca 661 tctcaggtga gaatctgatt ggcctgagca gagcccggcg cccccacaat gccatctttg 721 tcaactttga ggatgaggag gtgcccaagc agccactgga ggctgcagcc cagacgtgga 781 ggagagtctg cactaacccc gtggaccgga aggtggagga ggagctgagg aagctgtttg 841 acatccgtcc catctggtcc cgaaatgctg tcaaggccaa catcagcgtc cacccagaca 901 agctcaaggt cttgcttccc ttcatagcct attacatgat aacaggcccc tggcgcagcc 961 tatggattcg atttgggtat gacccccgaa aaaacccaga tgccaagatt tatcaagtcc 1021 tcgatttccg aatccgttgt ggaatgaaac acggttacgc ccccagtgac ttgccggtca 1081 aagcaaagcg cagcacctac aactacagcc tccccatcac cgtcaagaag acatccagcc 1141 agcttgtcac catgcatgac ctgaagcagg gcctgggccc gtcggggacg agtggtgctc 1201 ggaaaccagc ttccagcaag tacaagctca aggactctgt ctacatcttc cgggaagggg 1261 ccttgccacc ctatcggcag atgttctacc agttatgcga cttgaatgtg gaagagttgc 1321 agaagatcat tcaccgcaat gacggggcag agaatTCCTG CACAGAACGG GATGGGTGGT 1381 GCCTCCCCAA GACCAGCGAC GAGCTCAGGG ACACCATGTC CCTCATGATC CGGCAGACCA 1441 TCCGCTCCAA GAGGCCTGCT CTCTTTTCCA GCTCAGCCAA GGCTGATGGC GGAAAAGAGC 1501 AGCTGACGTA CGAGTCTGGG GAAGACGAGG AGGATGAGGA GGAGGAGGAA GAGGAGGAGG 1561 AGGACTTCAA GCCATCCGAC GGCAGTGAAA ACGAAATGGA GACAGAGATT CTGGACTATG 1621 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGAGG TATCCGCGAT AGTTCGACGG TAACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt