Construct: ORF TRCN0000465763
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010090.1_s317c1
- Derived from:
- ccsbBroadEn_11029
- DNA Barcode:
- AGGGTAACTTACCGACTCCGGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PGC (5225)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465763
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5225 | PGC | progastricsin | NM_001166424.1 | 32.4% | 30.8% | (many diffs) |
| 2 | human | 5225 | PGC | progastricsin | NM_002630.4 | 26.3% | 25% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 378
- ORF length:
- 312
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtggatggtg gtggtcttgg tctgcctcca gctcttGGAG GCAGCAGTGG 121 TCAAAGTGCC CCTGAAGAAA TTTAAGTCTA TCCGTGAGAC CATGAAGGAG AAGGGCTTGC 181 TGGGGGAGTT CCTGAGGACC CACAAGTATG ATCCTGCTTG GAAGTACCGC TTTGGTGACC 241 TCAGCGTGAC CTACGAGCCC ATGGCCTACA TGGATGCTGC CTACTTTGGT GAGATCAGCA 301 TCGGGACTCC ACCCCAGAAC TTCCTGGTCC TTTTTGACAC CGGCTCCTCC CCTCTTCCTC 361 TTGACCCTGC ACCCTCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 421 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 481 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGGGTAA CTTACCGACT 541 CCGGGCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt