Construct: ORF TRCN0000465774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008359.1_s317c1
- Derived from:
- ccsbBroadEn_07203
- DNA Barcode:
- CCGCGAGTGGATACGCACCAGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST8SIA2 (8128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465774
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8128 | ST8SIA2 | ST8 alpha-N-acetyl-neuramin... | NM_006011.4 | 99.8% | 99.7% | 621C>G;1033C>G |
| 2 | human | 8128 | ST8SIA2 | ST8 alpha-N-acetyl-neuramin... | NM_001330416.1 | 94.2% | 94.1% | 97_98ins63;558C>G;970C>G |
| 3 | human | 8128 | ST8SIA2 | ST8 alpha-N-acetyl-neuramin... | XM_017022642.1 | 88.9% | 84.8% | (many diffs) |
| 4 | mouse | 20450 | St8sia2 | ST8 alpha-N-acetyl-neuramin... | NM_009181.2 | 92% | 97.6% | (many diffs) |
| 5 | mouse | 20450 | St8sia2 | ST8 alpha-N-acetyl-neuramin... | XM_006540731.2 | 86.4% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1194
- ORF length:
- 1125
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagctgcag ttccggagct ggatgctggc cgcgctcacg ctgctcgtgg 121 tcttcctcat cttcgcagac atctcagaga tcgaagaaga aatcgggaat tcgggaggca 181 gaggtacaat cagatcagct gtgaacagct tacatagcaa atctaataga gctgaagttg 241 taataaacgg ctcctcatca ccagctgttg ttgacagaag taatgaaagc atcaagcaca 301 acatccagcc agcctcgtcc aaatggagac ataaccagac gctctctctg aggatcagga 361 agcagatttt aaagttcttg gatgctgaaa aggacatttc tgtcctaaag ggaaccctga 421 agcctggaga tattattcat tacatcttcg atcgagacag caccatgaat gtgtcccaga 481 acctctacga gctcctcccc aggacttcgc cactgaagaa taagcacttt gggacttgtg 541 ccatcgtggg caactcgggg gtcttgctga acagcggctg tgggcaggag attgacgccc 601 acagcttcgt catcaggtgc aacctggccc cagtacagga gtatgcccgg gatgtggggc 661 tcaagacaga cctggtaacc atgaacccgt cggtcatcca gcgggccttt gaggacttgg 721 tcaatgccac gtggcgggag aagctgctgc aacggctgca cagcctcaat ggcagcatcc 781 tgtggatccc tgccttcatg gcccggggcg gcaaggagcg tgttgagtgg gtcaacgagc 841 ttatcctgaa gcaccacgtc aacgtgcgca ctgcataccc ctcgctgcgc cTGCTGCACG 901 CCGTTCGCGG ATACTGGCTG ACCAACAAAG TCCACATCAA AAGACCCACC ACCGGCCTCT 961 TGATGTATAC CCTGGCCACA CGTTTCTGCA AACAAATCTA CCTCTACGGC TTCTGGCCCT 1021 TTCCGCTGGA TCAGAACCAG AACCCAGTCA AGTACCACTA TTATGACAGC CTCAAGTATG 1081 GCTACACCTC CCAGGCCAGC GCGCATACCA TGCCCTTGGA GTTTAAGGCC CTCAAGAGCC 1141 TACATGAGCA GGGGGCTTTG AAACTGACTG TCGGCCAGTG CGATGGGGCC ACGTTGCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAC CGCGAGTGGA TACGCACCAG CGCACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att