Transcript: Mouse NM_009181.2

Mus musculus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (St8sia2), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
St8sia2 (20450)
Length:
5376
CDS:
189..1316

Additional Resources:

NCBI RefSeq record:
NM_009181.2
NBCI Gene record:
St8sia2 (20450)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009181.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000110381 CCCGTCAAGTATCACTATTAT pLKO.1 1164 CDS 100% 15.000 12.000 N St8sia2 n/a
2 TRCN0000110380 GCCTGATAAATAATGGTGTTT pLKO.1 2810 3UTR 100% 4.950 3.960 N St8sia2 n/a
3 TRCN0000110382 CCTGGAGACATTATTCATTAT pLKO.1 543 CDS 100% 13.200 9.240 N St8sia2 n/a
4 TRCN0000035257 CAGCTTACATAGCAAATCTAA pLKO.1 326 CDS 100% 5.625 3.938 N ST8SIA2 n/a
5 TRCN0000110383 GCCTCAAGTATGGCTACACTT pLKO.1 1189 CDS 100% 4.950 3.465 N St8sia2 n/a
6 TRCN0000110384 GCAGACATCTCAGAGATCGAA pLKO.1 255 CDS 100% 3.000 2.100 N St8sia2 n/a
7 TRCN0000245796 TGTTGCTGCTGCTGTCGCCTT pLKO_005 38 5UTR 100% 0.720 0.432 N LRRC26 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009181.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07203 pDONR223 100% 92% 97.6% None (many diffs) n/a
2 ccsbBroad304_07203 pLX_304 0% 92% 97.6% V5 (many diffs) n/a
3 TRCN0000465774 CCGCGAGTGGATACGCACCAGCGC pLX_317 27.2% 92% 97.6% V5 (many diffs) n/a
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