Construct: ORF TRCN0000465782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013231.1_s317c1
- Derived from:
- ccsbBroadEn_00491
- DNA Barcode:
- CCCACAAGCCCAAGACATCACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF4EBP2 (1979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465782
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1979 | EIF4EBP2 | eukaryotic translation init... | NM_004096.5 | 100% | 100% | |
| 2 | mouse | 13688 | Eif4ebp2 | eukaryotic translation init... | NM_010124.2 | 91.3% | 95% | (many diffs) |
| 3 | mouse | 13688 | Eif4ebp2 | eukaryotic translation init... | XM_017313792.1 | 91.3% | 95% | (many diffs) |
| 4 | mouse | 13688 | Eif4ebp2 | eukaryotic translation init... | XM_017313793.1 | 91.3% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 426
- ORF length:
- 360
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ctcgtcagcc ggcagcggcc accagcccag ccagagccgc gccatcccca 121 cccgcaccgt ggccatcagc gacgccgcgc agctaccTCA TGACTATTGC ACCACGCCCG 181 GGGGGACGCT CTTCTCCACC ACACCGGGAG GAACTCGAAT CATTTATGAC AGAAAGTTTC 241 TGTTGGATCG TCGCAATTCT CCCATGGCTC AGACCCCACC CTGCCACCTG CCCAATATCC 301 CAGGAGTCAC TAGCCCTGGC ACCTTAATTG AAGACTCCAA AGTAGAAGTA AACAATTTGA 361 ACAACTTGAA CAATCACGAC AGGAAACATG CAGTTGGGGA TGATGCTCAG TTCGAGATGG 421 ACATCTGCCC AATTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCACAAGC CCAAGACATC ACTGCACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt