Construct: ORF TRCN0000465791
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003762.1_s317c1
- Derived from:
- ccsbBroadEn_05064
- DNA Barcode:
- CCTTCTTTCCCTGAGATTACTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC50 (152137)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465791
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152137 | CCDC50 | coiled-coil domain containi... | NM_174908.4 | 100% | 100% | |
| 2 | human | 152137 | CCDC50 | coiled-coil domain containi... | NM_178335.3 | 63.4% | 63.4% | 447_974del |
| 3 | mouse | 67501 | Ccdc50 | coiled-coil domain containi... | NM_026202.4 | 83.4% | 83.9% | (many diffs) |
| 4 | mouse | 67501 | Ccdc50 | coiled-coil domain containi... | NM_001025615.3 | 79.4% | 79.4% | (many diffs) |
| 5 | mouse | 67501 | Ccdc50 | coiled-coil domain containi... | XM_011246002.2 | 53.3% | 53.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 984
- ORF length:
- 918
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaagtcagc atcgaccagt ccaagctgcc tggagtcaag gaagtatgcc 121 gagattttgc tgtcctggag gaccacaccc tggctcacag cctgcaggaa caagagattg 181 agcatcattt ggcatcgaac gttcagcgga accgtttggt ccagcatgat ctccaggtgg 241 ctaagcagct ccaagaggaa gatctgaaag cgcaggccca gctccagaag cgctacaaag 301 accttgaaca acaagactgt gaaattgctc aggaaattca ggagaagctg gctattgagg 361 cagagagacg acgcattcag gagaagaagg atgaggacat agctcgcctt ttgcaagaaa 421 aggagttaca ggaagagaaa aagagaaaga aacactttcc agagttccct gcaacccgtg 481 cttatgcaga tagttactat tatgaagatg gaggaatgaa gccaagagtg atgaaagaag 541 ctgtatctac tccatcacga atggcccaca gggatcagga atggtatgat gctgaaattg 601 ccagaaaact gcaagaagaa gaacttttGG CTACCCAGGT GGACATGAGA GCCGCTCAAG 661 TAGCTCAAGA TGAAGAAATC GCTCGACTTC TAATGGCTGA AGAAAAGAAA GCTTACAAAA 721 AAGCCAAGGA GCGGGAGAAA TCATCTTTGG ACAAAAGAAA GCAAGACCCC GAGTGGAAGC 781 CAAAAACAGC TAAAGCAGCA AATTCCAAGT CAAAAGAGAG TGATGAACCT CACCATTCTA 841 AGAATGAAAG GCCAGCACGG CCACCACCAC CTATCATGAC AGATGGTGAA GATGCGGATT 901 ACACTCATTT TACAAACCAG CAGAGTTCCA CACGGCATTT CTCAAAATCA GAGTCCTCTC 961 ATAAAGGTTT TCATTACAAA CATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CTTCTTTCCC 1141 TGAGATTACT TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att