Transcript: Mouse NM_026202.4

Mus musculus coiled-coil domain containing 50 (Ccdc50), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Ccdc50 (67501)
Length:
7017
CDS:
134..1051

Additional Resources:

NCBI RefSeq record:
NM_026202.4
NBCI Gene record:
Ccdc50 (67501)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_026202.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000146450 CGACTTCTAATGGCTGAAGAA pLKO.1 752 CDS 100% 4.950 3.960 N CCDC50 n/a
2 TRCN0000184277 GCACAGTACAACATGGCATCT pLKO.1 985 CDS 100% 4.050 3.240 N Ccdc50 n/a
3 TRCN0000180733 GCAGCTGCTTTGCTGTTTATT pLKO.1 1475 3UTR 100% 15.000 10.500 N Ccdc50 n/a
4 TRCN0000328993 GCAGCTGCTTTGCTGTTTATT pLKO_005 1475 3UTR 100% 15.000 10.500 N Ccdc50 n/a
5 TRCN0000375162 ACAAGAGATTGAGCATCATTT pLKO_005 238 CDS 100% 13.200 9.240 N Ccdc50 n/a
6 TRCN0000375161 GGAAATTGCTCGACTTCTAAT pLKO_005 742 CDS 100% 13.200 9.240 N Ccdc50 n/a
7 TRCN0000328929 AGCTCCAGAAGCGCTACAAAG pLKO_005 348 CDS 100% 10.800 7.560 N Ccdc50 n/a
8 TRCN0000180106 CATGACCCTGAATGCAAGTTA pLKO.1 830 CDS 100% 5.625 3.938 N Ccdc50 n/a
9 TRCN0000328991 CATGACCCTGAATGCAAGTTA pLKO_005 830 CDS 100% 5.625 3.938 N Ccdc50 n/a
10 TRCN0000184397 CCAAGATTGACAGGCCATCAA pLKO.1 903 CDS 100% 4.950 3.465 N Ccdc50 n/a
11 TRCN0000129948 GAACAAGAGATTGAGCATCAT pLKO.1 236 CDS 100% 4.950 3.465 N CCDC50 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_026202.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05064 pDONR223 100% 83.4% 83.9% None (many diffs) n/a
2 ccsbBroad304_05064 pLX_304 0% 83.4% 83.9% V5 (many diffs) n/a
3 TRCN0000465791 CCTTCTTTCCCTGAGATTACTTGT pLX_317 39% 83.4% 83.9% V5 (many diffs) n/a
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