Construct: ORF TRCN0000465810
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003197.1_s317c1
- Derived from:
- ccsbBroadEn_03226
- DNA Barcode:
- ATACCTTGAAAATTCGGAATATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHCHD2 (51142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465810
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51142 | CHCHD2 | coiled-coil-helix-coiled-co... | NM_016139.4 | 100% | 100% | |
| 2 | human | 51142 | CHCHD2 | coiled-coil-helix-coiled-co... | NM_001320327.1 | 89.8% | 88.6% | 445_448delGGTA;458_504del |
| 3 | mouse | 14004 | Chchd2 | coiled-coil-helix-coiled-co... | NM_024166.6 | 82% | 84.9% | (many diffs) |
| 4 | mouse | 433806 | Chchd2-ps | coiled-coil-helix-coiled-co... | XM_485502.8 | 82% | 83.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gcgtggaagc cgaagccgca cctcccgcat ggcccctccg gccagccggg 121 ccccTCAGAT GAGAGCTGCA CCCAGGCCAG CACCAGTCGC TCAGCCACCA GCAGCGGCAC 181 CCCCATCTGC AGTTGGCTCT TCTGCTGCTG CGCCCCGGCA GCCAGGTCTG ATGGCCCAGA 241 TGGCAACCAC TGCAGCTGGC GTGGCTGTGG GCTCTGCTGT GGGGCACACA TTGGGTCACG 301 CCATTACTGG GGGCTTCAGT GGAGGAAGTA ATGCTGAGCC TGCGAGGCCT GACATCACTT 361 ACCAGGAGCC TCAGGGAACC CAGCCAGCAC AGCAGCAGCA GCCTTGCCTC TATGAGATCA 421 AACAGTTTCT GGAGTGTGCC CAGAACCAGG GTGACATCAA GCTCTGTGAG GGTTTCAATG 481 AGGTGCTGAA ACAGTGCCGA CTTGCAAACG GATTGGCCTA CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGAATACCT TGAAAATTCG GAATATGTAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt