Construct: ORF TRCN0000465818
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010679.1_s317c1
- Derived from:
- ccsbBroadEn_09522
- DNA Barcode:
- CAACAGGACTGTTGGACGCTCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BBS5 (129880)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465818
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129880 | BBS5 | Bardet-Biedl syndrome 5 | NM_152384.3 | 99.9% | 100% | 729A>G |
| 2 | mouse | 72569 | Bbs5 | Bardet-Biedl syndrome 5 (hu... | NM_028284.2 | 88.5% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1089
- ORF length:
- 1023
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggtgctggat gcgctttggg aggatcggga tgtccgtttc gacctgtccg 121 cgcagcaaat gaaaacaaga cctggagaag tccttattga ttgtttagat tccattgaag 181 acaccaaagg aaataatgga gatagaggta gactcttggt aacaaattta agaattctct 241 ggcactcttt ggcattatca agagtcaatg tttctgtcgg ttacaattgc atattgaata 301 ttacaacaag gactgctaac tctaaattac gaggccaaac tgaagctctc tatatactaa 361 caaaatgtaa cagtactcgt tttgaattta tatttacaaa tttggttcct ggaagcccta 421 gactttttac ttctgtgatg gcagtacaca gagcttatga aacttctaaa atgtatcgtg 481 attttaaatt aagaagtgca ctaattcaga acaagcaact aagactgttg ccacaagaac 541 atgtatatga taaaataaat ggagtttgga atttatccag tgatcagggc aatttaggaa 601 ccttttttat taccaatgtg agaattgtgt ggcatgcaaa tatgaatgat agttttaatg 661 tcagtatacc atatctgcaa attcgttcaa taaagattag agattcaaaa tttggtttag 721 ctcttgtcat agaaagctct cagcagagtg gtggatatgt tcttGGCTTT AAAATAGATC 781 CTGTGGAAAA ACTGCAAGAA TCAGTTAAGG AAATCAATTC ACTTCACAAA GTCTATTCTG 841 CCAGTCCCAT ATTTGGAGTT GATTATGAGA TGGAAGAAAA GCCCCAGCCG CTCGAAGCTC 901 TGACAGTCGA ACAAATTCAA GATGATGTAG AAATAGACTC TGATGGTCAC ACGGATGCTT 961 TTGTGGCTTA TTTTGCTGAT GGCAATAAGC AACAAGATCG TGAACCTGTA TTTTCAGAAG 1021 AACTGGGGCT TGCAATAGAG AAATTGAAGG ATGGATTCAC CCTACAGGGA CTTTGGGAAG 1081 TAATGAGTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1141 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1201 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAACAG GACTGTTGGA CGCTCACCAC 1261 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt