Construct: ORF TRCN0000465823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007666.1_s317c1
- Derived from:
- ccsbBroadEn_10711
- DNA Barcode:
- AGATATATCGTTTCAACCCTCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KYAT1 (883)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465823
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 105371063 | LOC105371063 | putative uncharacterized pr... | XM_011522781.1 | 27.9% | 6.7% | (many diffs) |
| 2 | human | 883 | KYAT1 | kynurenine aminotransferase 1 | NR_148224.1 | 12.8% | 1_1691del;1719G>T;2028_2613del | |
| 3 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020991.1 | 8.2% | 6.9% | (many diffs) |
| 4 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020992.1 | 8.2% | 6.9% | (many diffs) |
| 5 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020993.1 | 8.2% | 6.9% | (many diffs) |
| 6 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020994.1 | 8.2% | 6.9% | (many diffs) |
| 7 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020995.1 | 8.2% | 6.9% | (many diffs) |
| 8 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020996.1 | 8.2% | 6.9% | (many diffs) |
| 9 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020997.1 | 8.2% | 6.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 426
- ORF length:
- 336
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCGCCACCA CACCCGGCCA ATTTTTTTAT 121 TTTTGGTAGA GACGGGGTTT AGCCATGTTG GCCAGGCTGG TCTTGAACTC CTGACCTCAG 181 GTGATCCACC CGTCTCAGCC TCCCAAAGTG CTGGGATTAT AGGCGTGAGC CACCGCACCT 241 GGCCTTGTTC CTCTGACTTT GATGCAGCTC TAGGTGCTGG ATTGGAGCTA ACTCTCCTTC 301 CCCGCCTGGT CGTGGGAACC TCTCTGTCCC CTCCGGAGCC AAGGCCTGCT GGGCCACGAG 361 GCCGTGTTGA GACATGTCCT TCCTCCTCCC CAGGGCTTGG TGGCCATCCC TGTCTCCATC 421 TTCTATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGATATATC GTTTCAACCC TCACCACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt