Construct: ORF TRCN0000465840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010782.1_s317c1
- Derived from:
- ccsbBroadEn_10575
- DNA Barcode:
- ATATTCTCGCTCGCTTACTTGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALMS1P1 (200420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465840
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200420 | ALMS1P1 | ALMS1 pseudogene 1 | NR_003683.2 | 38.9% | (many diffs) | |
| 2 | human | 7840 | ALMS1 | ALMS1 centrosome and basal ... | NM_015120.4 | 4.1% | 3.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc catgaaggag tttcctggtt tgttcctttg gaaaatgtgg agtctagata 121 aaaagaagga aaacatgctc aagactcatg accctggcat ctcccggttg gaaccagtaa 181 ccaagaccaa gccgtggagg gagccactgt gggagcggaa ctggcagggg cagcacctgg 241 acagtcgggg ctacctggca ggcccaggca gagaggatgg cagaaaccCA CTGAAGCTGT 301 TTGTGAGAGC AACCCTGCAG GAATCGCTTC AGTTTCACAG ACCTGACTTC ATCTCCCACA 361 TTTGGGAGCG GATAAAGCGT CTGAAGTTAA TAGTCCAGGA GAGAAAGCTG CAGAGCATGT 421 TAAAGAGCGA GCGGGATGCG CTATTCGACA TTGACAGGGA ACGGCAGGGC CACCAGAATC 481 GCATGCGCCC ACTACCCAAG AGAGTCTTCC TGGCTGTCCA GAAGAACAAG CCTATCAGCA 541 AGAAGGAAAT GATTCAGAGG TCCAAACGGA CACACGCAGG AGGAACAGTA GCACAAAGGC 601 TTCCCGGTGC CTTGTGGGTG AAAGAAAGGC TTCCAGGGAA AGGAGAGAAA CACCCAGGGA 661 TCCCGGTGAC GTCGGGAAGC AGCACCCAGA CCATGTACCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 AATATTCTCG CTCGCTTACT TGGGAACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt