Construct: ORF TRCN0000465847
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005571.1_s317c1
- Derived from:
- ccsbBroadEn_09850
- DNA Barcode:
- AGCCATCATACTTAGTTCCACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL21 (219927)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465847
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219927 | MRPL21 | mitochondrial ribosomal pro... | NM_181514.2 | 98% | 98% | 0_1insATGGCGGCGGCC |
| 2 | human | 219927 | MRPL21 | mitochondrial ribosomal pro... | XR_247190.4 | 80.4% | (many diffs) | |
| 3 | human | 219927 | MRPL21 | mitochondrial ribosomal pro... | XM_005273823.4 | 66.5% | 64% | (many diffs) |
| 4 | human | 219927 | MRPL21 | mitochondrial ribosomal pro... | NM_181515.2 | 57.4% | 57.4% | 0_1ins267 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggccatg gcagcatctt ccctgacggt caccttaggg cggctggcgt 121 ccgcgtgcag ccacagcatc ctgagacctt cggggcccgg agcagcctcc ctttggtctg 181 cttctcgaag gttcaattca cagagcactt catatctacc aggatatgtt cctaaaacat 241 ccctgagttc accaccttgg ccagaagttg ttctgccaga ccCAGTTGAG GAGACCAGAC 301 ACCATGCAGA GGTCGTGAAG AAGGTGAATG AGATGATCGT CACGGGGCAG TATGGCAGGC 361 TCTTTGCCGT GGTGCACTTT GCCAGCCGCC AGTGGAAGGT GACCTCTGAA GACCTGATCT 421 TAATTGGAAA TGAACTAGAC CTTGCGTGTG GAGAGAGAAT TCGACTGGAG AAGGTCCTGC 481 TGGTTGGGGC AGACAACTTC ACGCTGCTTG GCAAGCCACT CCTCGGAAAG GATCTTGTTC 541 GAGTAGAAGC CACAGTCATT GAAAAGACAG AATCATGGCC AAGAATCATT ATGAGATTCA 601 GGAAAAGGAA AAACTTCAAG AAGAAAAGAA TCGTCACGAC CCCGCAGACT GTCCTCCGGA 661 TAAACAGCAT TGAGATTGCT CCGTGTTTGT TGTGCCTAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 841 CCATCATACT TAGTTCCACC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt