Construct: ORF TRCN0000465853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006483.1_s317c1
- Derived from:
- ccsbBroadEn_04857
- DNA Barcode:
- ACGCTTACCGCACCAGTAAGAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM43 (129868)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129868 | TRIM43 | tripartite motif containing 43 | NM_138800.3 | 100% | 100% | |
| 2 | human | 653192 | TRIM43B | tripartite motif containing... | NM_001164464.1 | 99.4% | 98.6% | (many diffs) |
| 3 | human | 653192 | TRIM43B | tripartite motif containing... | XM_011511669.1 | 97.2% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1404
- ORF length:
- 1338
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctcagacttc tcacatgcct tccagaagga actcacctgc gtcatctgtt 121 tgaactacct ggtagaccct gtcaccatct gctgtgggca cagcttctgt aggccctgtc 181 tctgcctttc gtgggaggaa gcccaaagtc ctgcaaactg ccctgcatgc agggaaccat 241 caccgaaaat ggacttcaaa accaatattc ttctgaagaa tttagtgacc attgccagaa 301 aagccagtct ctggcaattc ctgagctctg agaaacaaat atgtgggacc cataggcaaa 361 caaagaagat gttctgtgac atggacaaga gtctcctctg cttgctgtgc tccaactctc 421 aggagcacgg ggctcacaaa caccatccca tcgaagaggc agctgaggaa caccgggaga 481 aactcttaaa gcaaatgagg attttatgga aaaagattca agaaaatcag agaaatctat 541 atgaggaggg aagaacagcc ttcctctgga ggggcaatgt ggttttacgg gcacagatga 601 tcaggaatga gtataggaag ctgcatccgg ttctccataa ggaagaaaaa caacatttag 661 agagactgaa caaggaatac caagagattt ttcagcaact ccagagaagt tgggtcaaaa 721 tggatcaaaa gagtaaacac ttgaaagaaa tgtatcagga actaatggaa atgtgtcata 781 aaccagatgt ggagctgctc caggatttgg gagacatcgt ggcaaggagt gagtccgtgc 841 tgctgcacat gccccagcct gtgaatccag agctcactgc aggacccatc actggactgg 901 tgtacaggct caaccgcttc cgagtggaaa tttccttcca ttttgaagta accaatcaca 961 atatcaggct ctttgaggat gtgagaagtt ggatgtttag acgtggacct ttgaattctg 1021 acagatctga ctattttgct gcatggGGAG CCAGGGTCTT CTCCTTTGGG AAACACTACT 1081 GGGAGCTGGA TGTGGACAAC TCTTGTGACT GGGCTCTGGG AGTCTGTAAC AACTCCTGGA 1141 TAAGGAAGAA TAGCACAATG GTTAACTCTG AGGACATATT TCTTCTTTTG TGTCTGAAGG 1201 TGGATAATCA TTTCAATCTC TTGACCACCT CCCCAGTGTT TCCTCACTAC ATAGAGAAAC 1261 CTCTGGGCCG GGTTGGTGTG TTTCTTGATT TTGAAAGTGG AAGTGTGAGT TTTTTGAATG 1321 TCACCAAGAG TTCCCTCATA TGGAGTTACC CAGCTGGCTC CTTAACTTTT CCTGTCAGGC 1381 CTTTCTTTTA CACTGGCCAC AGATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGCCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CGCTTACCGC 1561 ACCAGTAAGA ATTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att