Construct: ORF TRCN0000465860
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009880.1_s317c1
- Derived from:
- ccsbBroadEn_16136
- DNA Barcode:
- TTATTTGGGAGCACATGAATTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEATR9 (256957)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465860
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_017024424.2 | 42.1% | 38.3% | (many diffs) |
| 2 | human | 256957 | HEATR9 | HEAT repeat containing 9 | NM_152781.4 | 36.7% | 33.5% | (many diffs) |
| 3 | human | 256957 | HEATR9 | HEAT repeat containing 9 | NR_135630.2 | 31.2% | (many diffs) | |
| 4 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_005278233.2 | 30.8% | 27.6% | (many diffs) |
| 5 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_011524586.2 | 30.8% | 27.6% | (many diffs) |
| 6 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XR_934432.3 | 30% | (many diffs) | |
| 7 | human | 256957 | HEATR9 | HEAT repeat containing 9 | NM_001321395.2 | 29.8% | 26.5% | (many diffs) |
| 8 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XR_934433.1 | 29.4% | (many diffs) | |
| 9 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XR_934434.1 | 24.5% | (many diffs) | |
| 10 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_005278235.1 | 23.8% | 20.7% | (many diffs) |
| 11 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XR_254513.3 | 23.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctatgaaaaa tcaactgata tctctgatgt ctccaggtca atgttcctgt 121 acccatggct ggaatatcca gacaagacca aagaactcag aaaagccatg gctcctgttc 181 atctgccctt gtcctgctac cagatgccaa aggaagagtt tcccccaagt ccagagtgct 241 ggaggcagca tccgagcaag ccaaactcag tcccgtactg ctacttcaag aaacctgaga 301 tctacacgca ctggcacgac ctgtatgatc agcgagagga aagggaggct gagaagatgt 361 tgaggaaaat gagagatgac tgtaggtaca tCAAAGAGGT ACATCAAACC CACATCAAAA 421 TGTTCCATCT CCCAATGAGC AAGCTGACTA TAAAATCTGA GATGCGATCC AGGCCCTTAG 481 AGCCTACCCA GGACCCCCTG AAGTGGCAAA GATTAAGGGA ACTCACAAAA AGCCTGGAAT 541 CTCCCAGAGA GGATGAGCAG TTCTATGCAG CACAGGCTCT GGGATGCTTA CGCATCAGTG 601 ACAAGTTTGT CATGGAGGCA CTACAGCAGG TGGTGAGTCT GAGAACTGCT GGAGTTCAGG 661 AAAGAAGGAA ACAGGGTCTG AGATGTGTGG CATGTGGGCA CAGACGAGGC TCCCCGGGGT 721 GCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGATT ATTTGGGAGC ACATGAATTC AAACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt