Construct: ORF TRCN0000465861
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007310.1_s317c1
- Derived from:
- ccsbBroadEn_14190
- DNA Barcode:
- ACCTATGGCCATGGACCGGCTGAA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P3H2 (55214)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465861
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55214 | P3H2 | prolyl 3-hydroxylase 2 | NM_001134418.1 | 99.8% | 99.2% | 153C>T;1566delC |
| 2 | human | 55214 | P3H2 | prolyl 3-hydroxylase 2 | XM_011512955.1 | 99.8% | 99.2% | 153C>T;1566delC |
| 3 | human | 55214 | P3H2 | prolyl 3-hydroxylase 2 | NM_018192.4 | 74.3% | 73.8% | 1_543del;696C>T;2109delC |
| 4 | mouse | 210530 | P3h2 | prolyl 3-hydroxylase 2 | NM_173379.2 | 64.1% | 68.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1647
- ORF length:
- 1581
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aatgcagcag aacattgaga attacagggc gacagctggt gttgaagcat 121 tgcagttggt agacagagaa gccaagccac acatggagag ttacaatgca ggagttaaac 181 attatgaggc tgatgacttt gagatggcta tcaggcattt cgaacaagcc ttaagagaat 241 atttcgttga agatacagaa tgccggaccc tatgtgaggg gcctcagaga tttgaagaat 301 atgagtattt agggtataag gctggtctgt atgaagctat tgcagatcac tacatgcagg 361 tgcttgtttg tcagcatgaa tgtgtgaggg aacttgccac ccgccctggc cgcctctctc 421 ccatcgagaa ttttcttcct ctgcactatg attacctaca gtttgcctac tatcgagttg 481 gtgagtatgt gaaagccctg gagtgtgcca aagcctatct tctatgccat ccagatgatg 541 aggatgtcct agacaatgtg gattactatg agagtctgct ggatgatagc attgacccgg 601 catccattga ggccagagag gatttaacaa tgtttgtgaa acgtcataag ctggagtctg 661 agctgataaa atcagctgca gaaggtctgg ggttttcata cactgaaccg aattattgga 721 tcagatatgg aggacgacag gatgagaatc gggtcccttc aggagtgaac gtagagggag 781 cagaagttca tggattctca atgggaaaaa agctatcacc caagatagat cgagacctaa 841 gagaaggtgg tcctctactc tatgagaaca tcacattcgt ctacaactcg gagcagctga 901 acgggactca gcgggttctc ctggataacg tcctgtcgga agaacagtgc cgggagctcc 961 acagcgtggc cagtggaatc atgcttgttg gtgatggata cagaggaaaa acttcacccc 1021 atacacccaa tgaaaagttt gaaggtgcaa ctgtcctgaa agcactcaaa tctggttatg 1081 aaggtcgagt cccactgaag agcgctcgtc tgttttatga catcagcgaa aaggctcgaa 1141 ggattgtaga atcttatttt atgctgaact caactctgta tttttcctat acacacatgg 1201 tctgccgaac agccctgtct ggtcagcagg atagaagaaa tgacctcagt catcccatcc 1261 atgctgacaa ctgtttgttg gatccagagg ccaacgaatg ctggaaggag ccTCCTGCTT 1321 ACACATTTCG AGACTATAGT GCTCTCCTAT ATATGAATGA TGACTTTGAA GGAGGAGAAT 1381 TCATATTCAC AGAGATGGAT GCTAAGACTG TGACTGCCTC TATAAAACCA AAATGTGGGC 1441 GCATGATCAG CTTCTCATCT GGAGGAGAGA ACCCTCATGG GGTGAAGGCA GTCACCAAGG 1501 GAAAGAGGTG TGCTGTGGCT CTGTGGTTCA CCTTGGACCC ACTTTATAGA GAATTGGAGC 1561 GAATACAGGC TGATGAAGTG ATTGCAATTC TGGATCAAGA ACAGCAAGGG AAGCATGAAC 1621 TGAATATCAA CCTAAAGATG AGCTATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG 1681 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 1741 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AACCTATGGC 1801 CATGGACCGG CTGAAACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 1861 agatt