Construct: ORF TRCN0000465862
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000698.1_s317c1
- Derived from:
- ccsbBroadEn_08207
- DNA Barcode:
- AAACGTCGCGTCCATGCTGTTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALHM2 (51063)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465862
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51063 | CALHM2 | calcium homeostasis modulat... | NM_015916.5 | 99.8% | 99.6% | 13A>N |
| 2 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_006717880.2 | 99.8% | 99.6% | 13A>N |
| 3 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_006717883.2 | 99.8% | 99.6% | 13A>N |
| 4 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_011539848.2 | 99.8% | 99.6% | 13A>N |
| 5 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_017016306.1 | 99.8% | 99.6% | 13A>N |
| 6 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_017016307.1 | 99.8% | 99.6% | 13A>N |
| 7 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_024448034.1 | 99.8% | 99.6% | 13A>N |
| 8 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_006717884.4 | 60.5% | 57.5% | 13A>N;539_540insCAGGTATGAGTCC;588_589ins368 |
| 9 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_017016308.2 | 60.5% | 57.5% | 13A>N;539_540insCAGGTATGAGTCC;588_589ins368 |
| 10 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_017016309.2 | 60.5% | 57.5% | 13A>N;539_540insCAGGTATGAGTCC;588_589ins368 |
| 11 | human | 51063 | CALHM2 | calcium homeostasis modulat... | XM_024448035.1 | 60.5% | 57.5% | 13A>N;539_540insCAGGTATGAGTCC;588_589ins368 |
| 12 | human | 51063 | CALHM2 | calcium homeostasis modulat... | NR_046344.2 | 53.4% | 1_473del;486A>N;1443_1811del | |
| 13 | human | 51063 | CALHM2 | calcium homeostasis modulat... | NR_024552.2 | 52.7% | (many diffs) | |
| 14 | mouse | 72691 | Calhm2 | calcium homeostasis modulat... | NM_133746.5 | 87% | 91.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agccctgntc gcagagaact tccgcttcct gtcacttttc ttcaagagca 121 aggatgtgat gattttcaac ggcctggtgg cactgggcac ggtgggcagc caggagctgt 181 tctctgtggt ggccttccac tgcccctgct cgccggcccg gaactacctg tacgggctgg 241 cggccatcgg cgtgcccgcc ctggtgctct tcatcattgg catcatcctc aacaaccaca 301 cctggaacct cgtggccgag tgccagcacc ggaggaccaa gaactgctcc gccgccccca 361 ccttcctcct tctaagctcc atcctgggac gtgcggctgt ggcccctgtc acctggtctg 421 tcatctccct gctgcgtggt gaggcttatg tctgtgctct cagtgagttc gtggaccctt 481 cctcactcac ggccagggaa gagcacttcc catcagccca cgccactgaa atcctggcca 541 ggttcccctg caaggagaac cctgacaacc tgtcagactt ccgggaggag gtcagccgca 601 ggctcaggta tgagtcccag ctctttggat ggctgctcat cggcgtggtg gccatcctgg 661 tgttcctgac caagtgcctc aagcattact gctcaccact cagctaccgc caggaggccT 721 ACTGGGCGCA GTACCGCGCC AATGAGGACC AGCTGTTCCA GCGCACGGCC GAGGTGCACT 781 CTCGGGTGCT CGCTGCCAAC AATGTGCGCC GCTTCTTTGG CTTTGTGGCG CTCAACAAGG 841 ATGATGAGGA ACTGATTGCC AACTTCCCAG TGGAAGGCAC GCAGCCACGG CCACAGTGGA 901 ATGCCATCAC CGGCGTCTAC TTGTACCGTG AGAACCAGGG CCTCCCACTC TACAGCCGCC 961 TGCACAAGTG GGCCCAGGGT CTGGCAGGCA ACGGCGCGGC CCCTGACAAC GTGGAGATGG 1021 CCCTGCTCCC CTCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAACGTCGCG TCCATGCTGT 1201 TGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt