Construct: ORF TRCN0000465863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016568.1_s317c1
- Derived from:
- ccsbBroadEn_12275
- DNA Barcode:
- CCGTGCAACTTTGTCGACCACATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMBRD1 (55788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55788 | LMBRD1 | LMBR1 domain containing 1 | NM_001363722.2 | 40.1% | 34.1% | (many diffs) |
| 2 | human | 55788 | LMBRD1 | LMBR1 domain containing 1 | NM_001367271.1 | 40.1% | 34.1% | (many diffs) |
| 3 | human | 55788 | LMBRD1 | LMBR1 domain containing 1 | NM_001367272.1 | 40.1% | 34.1% | (many diffs) |
| 4 | human | 55788 | LMBRD1 | LMBR1 domain containing 1 | NM_018368.4 | 34.7% | 29.5% | (many diffs) |
| 5 | mouse | 68421 | Lmbrd1 | LMBR1 domain containing 1 | NM_001310483.1 | 34.8% | 32.6% | (many diffs) |
| 6 | mouse | 68421 | Lmbrd1 | LMBR1 domain containing 1 | XM_017322292.1 | 30.3% | 28.5% | (many diffs) |
| 7 | mouse | 68421 | Lmbrd1 | LMBR1 domain containing 1 | NM_026719.2 | 30.2% | 28.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 630
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggcagctata acttacacag cctatggcat gtctgcgtta cctttaaatc 121 tgataaaagg cactagaagc gctgcttatg aacgtttgga aaacactgaa gacattgaag 181 aagtagaaca acacattcaa acgattaaat caaaaagcaa agatggtcga cctttgccag 241 caagggataa acgcgcctta aaacaattTG AAGAAAGGTT ACGAACACTT AAGAAGAGAG 301 AGAGGCATTT AGAATTCATT GAAAACAGCT GGTGGACAAA ATTTTGTGGC GCTCTGCGTC 361 CCCTGAAGAT CGTCTGGGGA ATATTTTTCA TCTTAGTTGC ATTGCTGTTT GTAATTTCTC 421 TCTTCTTGTC AAATTTAGAT AAAGCTCTTC ATTCAGCTGG AATAGATTCT GGTTTCATAA 481 TTTTTGGAGC TAACCTGAGT AATCCACTGA ATATGCTTTT GCCTTTACTA CAAACAGAAT 541 TCGAAATATT GGCATATGGT TCTTTTGGAT TAGATTATAT AAAATCAGAA GAGGTAGAAC 601 CAGGCCCTAA GCACTCCTTT TTCTCTGCAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCGTG 781 CAACTTTGTC GACCACATCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt